用荧光标记MVR-PCR方法研究中国汉族人群DYF155S1基因座多态性  

Studying the Polymorphism at DYF155S1 Locus in Chinese Han Population by MVR-PCR Marked with Fluorescence

作　　者：庾蕾[1] 伍新尧[1] 黄艳梅[1] 蔡贵庆[1] 许传超[1] 童大跃[1] 

机构地区：[1]中山大学中山医学院法医系,广州510089

出　　处：《Acta Genetica Sinica》2003年第1期15-19,共5页

基　　金：广东省自然科学基金资助项目(编号 97-12 8);中山医科大学"2 11工程"重点学科建设课题基金资助项目(编号 42 0 90 0 8)

摘　　要：建立简便、实用的DYF15 5S1基因座基因分型的银染和荧光标记半自动分析方法 ,对中国汉族 15 5个无关男性个体血样本DNA进行分型 ,两种方法分型结果一致。 15 5人共检出 66种等位基因 ,有 3 8个等位基因仅出现 1次。根据图谱中第一条DNA片段及DNA条带数从小至大命名 ,频率最高的为 18和 2 2号等位基因 ,其第一条DNA片段大小为 180bp ,DNA条带数为连续的 16和 17个 ,频率均为 0 0 65。这一位点的基因多样性 (h)为 0 9789。 15 5人中有 2 5人表现为连续DNA谱带中有 1个或 2个DNA条带的丢失 (nullrepeat) ,序列分析证明丢失的DNA条带位置对应于 3型核心序列。结果表明 ,本文建立的分析方法能很好地揭示DYF15 5S1基因座 5′端多态性 ,是目前仅作1次PCR能获得个体Y染色体多态信息较高的技术。用本方法建立的中国汉族人群DYF15 5S1基因座等位基因频率资料 ,为群体遗传学研究及法医学应用提供了基础资料。The simple and useful genotyping methods of DYF155S1 locus by silver staining and fluorescence detection have been established.The blood samples from 155 unrelated males in Chinese Han population were typed by these two methods respectively and the same results were obtained.Among the 155 samples 66 alleles were found,out of them 38 were observed once only.The most frequent alleles named 18 or 22,which frequency was 0 065,the size of their first DNA fragments was 180 bp and the number of fragments was 16 or 17.The gene diversity ( h ) was 0 9789.Out of the 155 samples,25 samples had one or two bands lost (null repeat) among the successive bands.It was demonstrated by sequencing that the position of the lost bands corresponded to type 3 repeats.Our results indicated that the method,which revealed the 5′ end diversity of DYF155S1 locus,was a technique that could obtain the most Y-specific polymorphic information only by one PCR reaction.The allele frequencies of DYF155S1 locus in Chinese Han population provided the basic data for the study of population genetics and forensic practices.

关 键 词：荧光标记MVR-PCR方法 中国 汉族人群 DYF155S1基因座 MSY1 DNA多态性 

分 类 号：Q987[生物学—遗传学]