Senescence—like changes induced by expression of p21^Waf1／Cip1 in NIH3T3 cell line  

作　　者：X1CHEN WEIZHANG 等 

机构地区：[1]CollegeofLifeSciences,PekingUniversity,Beijing100871,China [2]TheKeyLaboratoryofCellProliferationandRegulationBiologyoftheMinistryofEducation,CollegeofLifeSciences,BeijingNormalU

出　　处：《Cell Research》2002年第3期229-233,共5页细胞研究（英文版）

基　　金：国家重点基础研究发展计划(973计划)

摘　　要：P21Waf1/Cip1 is a potent cyclin-dependent kinase inhibitor. As a downstream mediator of p53, p21Waf1/Cip1 involves in cell cycle arrest, differentiation and apoptosis. Previous studies in human cells provided evidence for a link between p21Waf1/Cip1 and cellular senescence. While in murine cells, the role of p21Waf1/Cip1 is indefinite. We explored this issue using NIH3T3 cells with inducible p21Waf1/Cip1 expression. Induction of p21Waf1/Cip1 triggered G1 growth arrest, and NIH3T3-p21 cells exhibited morphologic features, such as enlarged and flattened cellular shape, specific to the senescence phenotype. We also showed that p21Waf1/Cip1-transduced NIH3T3 cells expressed β-galactosidase activity at pH 6.0, which is known to be a marker of senescence. Our results suggest that p21Waf1/Cip1 can also induce senescence-like changes in murine cells.

关 键 词：P21WAF1/CIP1 SENESCENCE inducible expression cell cycle arrest 

分 类 号：Q255[生物学—细胞生物学]