人慢性胃炎胃粘膜内胃泌素及其mRNA的表达和意义  

EXPRESSION AND SIGNIFICANCE OF GASTRIN AND ITS mRNA IN THE MUCOSA OF CHRONIC GASTRITIS

作　　者：周凡[1] 葛振华[1] 武一曼[2] 唐福康[1] 王若愚[1] 周维湛[1] 

机构地区：[1]福建省中医药研究院 [2]福建中医学院,福州350003

出　　处：《中国组织化学与细胞化学杂志》2002年第4期421-424,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：福建省卫生厅科研基金资助课题(No.96057)

摘　　要：目的　探讨慢性胃炎胃粘膜对细胞内胃泌素 (G)及其mRNA的表达和意义。方法　标本均来自活检的胃窦部粘膜组织 ,用免疫细胞化学和原位杂交技术。结果　对照组G细胞及GmRNA阳性细胞数 ,与浅表性胃炎组比较无显著性差异 (P >0 0 5 ) ,而与萎缩性胃炎组比较则有显著性差异 (P <0 0 1) ,不论对照组还是胃炎组的G细胞数量与GmRNA阳性细胞均有平行关系。结论　证明浅表性和萎缩性胃炎的胃泌素基因的转录和蛋白表达功能保存完好 ,因此检测G细胞的数量及GmRNA不仅可以帮助了解胃炎的萎缩程度 ,而且能判断临床疗效。? Objective To investigate the expression and significance of gastrin and its mRNA in the mucosa of chronic gastritis.Method All samples were obtained by biopsy during gastroscopy. Immunocytochemical and in situ hybridization techniques were used. Result The number of G cells and G mRNA+ cells were higher in the control group than in the superficial and the atrophic gastritis groups. There was a significant difference (P<0.01), between the control group and the atrophic gastritis group but no significant difference (P>0.05) between the control and the superficial gastritis groups. The number of G cells was consistently parallel with that of G mRNA+ cells whether in the control group or in the gastritis groups. Conclusion The transcription of gastrin gene and the expression of protein were preserved well in both superficial and atrophic gastritis. Thus the detection of G cells or G mRNA in the mucosa of chronic gastritis might be a valuable marker for evaluating the grade of atrophic gastritis and clinical therapeutic effects. 〔

关 键 词：慢性胃炎 G细胞 G mRNA阳性细胞 免疫细胞化学 原位杂交 人 

分 类 号：R573.3[医药卫生—消化系统]