PTEN蛋白在子宫内膜癌中的表达及其临床意义  

DETECTION OF PTEN PROTEIN EXPRESSION AS DIAGNOSTIC MARKER FOR EARLIEST ENDOMETRIAL CANCER AND PRECANCER

作　　者：高庆蕾[1] 叶飞[1] 邢辉[1] 李辅军 卢运萍[1] 马丁[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2002年第4期425-428,52,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金资助项目(No.39840009,39870765);国家杰出青年基金资助项目(No.30025017)

摘　　要：目的　探讨抑癌基因PTEN产物PTEN蛋白在由正常子宫内膜过渡至子宫内膜癌过程中的表达缺失情况及其临床意义。方法　用SP免疫组化法测定 73例子宫内膜癌、 2 5例子宫内膜非典型增生、 71例子宫内膜增殖症和 31例正常子宫内膜组织中PTEN蛋白的表达 ,并与组织学类型、组织学分级、肌层浸润深度和临床分期等生物学行为进行相关分析。结果　PTEN蛋白在子宫内膜腺癌和癌前病变中的表达缺失率分别为 6 6 6 7%和 76 0 0 % ,表达缺失率与组织学类型(P <0 0 0 5 )和组织学分级有关 (P <0 0 5 ) ,与子宫内膜癌肌层浸润深度和临床分期无关 (P >0 0 5 )。结论　PTEN表达缺失是子宫内膜癌发生的早期分子事件 ,PTEN蛋白表达缺失是早期诊断子宫内膜癌及癌前病变较好的生物标志。? Objective To study the clinical significance of PTEN expression in the transition from normal endometrium to endometrial cancers and precancers.Method PTEN expression was detected by SP immunohistochemical methods on paraffin sections from 73 cases of endometrial carcinoma, 25 of endometrial intraepithelial neoplasia, 71 of endometrial neoplasia and 31 of normal endometrium. Result Loss of PTEN protein expression was found in 66.67% endometrioidadenocarcinoma and 76.00% endometrial intraepithelial neoplasia. In endometrial carcinoma the loss of PTEN expression was significantly related to the tissue type (P<0.005) and the cell grade(P<0.05), but not related to the depth of myometrium invasion and the clinical stage(P>0.05). Conclusion The loss of PTEN expression is an early event in endometrial tumorigenesis. The detection of PTEN protein may be used as a diagnostic biomarker for the earliest endometrial precancers . 〔

关 键 词：子宫内膜肿瘤 蛋白 PTEN 基因表达 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]