胚胎期下颌髁突软骨中糖胺多糖、纤维粘连蛋白和层粘连蛋白的表达  

EXPRESSION OF GLYCOSAMINOGLYCAN, FIBRONECTIN AND LAMININ IN FETAL MANDIBULAR CONDYLAR CARTILAGE

作　　者：陈新明[1] 王丽[1] 陈卫民[2] 汪说之[1] 李原[1] 

机构地区：[1]武汉大学口腔医学院,武汉430079 [2]华中科技大学同济医学院附属同济医院,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2002年第4期443-446,514,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：湖北省教委资助课题(No.97052)

摘　　要：目的　观察不同时期胎儿髁突软骨中糖胺多糖、纤维粘连蛋白和层粘连蛋白的表达。方法　应用组织化学和免疫组织化学SP法检测 32例 13周～ 33周 (A组 13～ 17周 ,B组 18～ 2 2周 ,C组 2 3～ 2 7周 ,D组 2 8～ 33周 )胎儿髁突软骨中糖胺多糖、纤维粘连蛋白和层粘连蛋白 ,并行体视学图象分析。结果　髁突软骨中AB PAS在各组的灰度及积分光密度均无显著性差异 (P >0 . 0 5 )。FN、LN阳性表达位于软骨细胞外基质 ,增殖层和成软骨细胞层呈强表达 ,尤其在两层交界处 ,而肥大软骨细胞层中明显减弱。体视学分析表明LN在A组表达最强 ,其灰度及积分光密度均值与B、D组间均有显著性差异 (P <0 . 0 5 )。〔灰度 :A组 175 . 0 18± 8. 30 1、B组 187 16 2± 13 6 44、D组 190 5 5 0± 6 46 0 ;积分光密度 :A组 2 739± 0 799,B组 2 0 11± 0 85 5 ,D组 1 5 33± 0 42 7,均存在显著性差异 (P <0 0 5 )。FN在B组表达最强 ,灰度与A、C、D三组间存在显著性差异 (P <0 0 5或P <0 0 1) ;积分光密度与A、D组间有显著性差异 (P <0 0 5 )。〔灰度 :B组 173 0 89± 8 40 7、A组 182 0 45± 10 138、C组 188 70 1± 8 0 94、D组 195 417± 4 82 7;积分光密度 :A组 1 314± 0 86 9、B组 4 332± 2 5 37、D组 2? Objective To observe glycosaminoglycan (GAG), fibronectin (FN) and laminin (LN) in fetal mandibular condylar cartilage at different embryonic stages. Method The mandibular condylar cartilage of 32 human fetuses aged from 13 to 33 weeks of gestation were utilized. They were divided into four groups: group A (13～17wks), group B (18～22wks), group C (23～27wks) and group D (28～33wks) according to gestational age. Histochemistry and immunohistochemical SP method were used and two parameters-average gray degree (AGD) and integral optical density (IOD)-were analyzed statistically. Result The results showed no significant differences in the AGD and IOD of GAG among the four groups. The expression of FN and LN was in the extracellular matrix, very high in the proliferating and chondroblastic zones, especially in the area between these two zones, but obvious low in the hypertrophic zone. The highest expression of LN was in group A, and there were significant differences in both AGD and IOD between group A and groups B and D (P<0.05). The highest expression of FN was in group B, and there were significant differences in GAD between group B and the other three groups (P<0.05) and in IOD between group B and groups A and D (P<0.05). Conclusion The level of GAG was constant in condylar cartilage. In contrast, the levels of FN and LN varied during the development of fetal mandibular condylar cartilage. 〔

关 键 词：髁突软骨 糖胺多糖 纤维粘连蛋白 层粘连蛋白 胎儿 组织化学 免疫组织化学 

分 类 号：R780.2[医药卫生—口腔医学]