背根切断对脊髓后角Ⅱ板层BDNF,NT-3表达的影响——免疫组织化学研究  

EFFECT OF DORSAL ROOT RHIZOTOMY ON THE EXPRESSION OF BDNF AND NT-3 IN SPINAL DORSAL HORN LAMINA Ⅱ

作　　者：张晓[1] 刘芬[2] 王廷华[2] 袁源[2] 吴林艳[2] 秦亚辉[2] 

机构地区：[1]第三军医大学成都军医学院实验技术教研室,成都610083 [2]昆明医学院神经科学研究所,昆明650031

出　　处：《中国组织化学与细胞化学杂志》2002年第4期462-464,共3页Chinese Journal of Histochemistry and Cytochemistry

基　　金：云南省自然科学基金资助(No.99C0006R)

摘　　要：目的　采用免疫组织化学技术探讨切断背根 (L1)后脊髓Ⅱ板层脑源性神经营养因子 (BDNF)和神经营养因子 3 (NT 3)表达的变化。方法　将成年雄猫 5只行单侧L1背根切断术 (对侧为非手术侧 )。术后 5天取L1脊髓制作2 0 μm厚冰冻切片 ,用BDNF及NT 3抗体分别进行免疫组化染色。观察BDNF、NT 3免疫阳性反应物在脊髓的分布 ,计数单位面积内Ⅱ板层BDNF阳性膨体密度及NT 3阳性细胞数。结果用t检验进行统计分析。结果　BDNF样免疫反应物在Ⅱ板层主要分布于神经膨体 ,NT 3样免疫反应物在神经元及胶质细胞均有分布。背根切断后 ,手术侧Ⅱ板层BDNF阳性膨体数量明显较非手术侧者减少 (P <0 0 1)。而手术侧Ⅱ板层NT 3阳性神经元及胶质细胞数量则较非手术侧者明显增加(P <0 0 1)。结论　背根切断后脊髓Ⅱ板层BDNF ,NT 3的表达发生不同变化。BDNF减少 ,而NT 3表达增多。提示BD NF和NT 3在脊髓损伤修复中的不同作用。? Objective To explore the changesof BDNF and NT-3 expression in spinal dorsal horn lamina Ⅱ after dorsal root(L 1) rhizotomy. Method Five male adult cats underwentunilateral dorsal root(L 1) rhizotomy. All the animals were sacrificed at day 5 after the operation,andthe L 1 spinal segmentswere taken and sectioned into slices of 20μm in thickness. The sections were stained withimmunohistochemicalmethod (BDNF antibody 1∶500,Santa Cruz; NT-3 antibody 1∶1500, Santa Cruz). The density of BDNF positive varicosities and the number of NT-3 positive neurons and glial cells in lamina Ⅱ were counted and analyzed by statistical method. Result At day 5 after L 1 dorsal root rhizotomy, the density of BDNF positive varicosities in laminaⅡ of the operated side decreased significantly when compared with that of the unoperated side(P<0.01). On the contrary, the number of NT-3 positive neurons and glial cells in laminaⅡ was much greater on the operated side than on the unoperated side(P<0.01). Conclusion Our results suggest that the reactionsof BDNF and NT-3 in spinal lamina II following dorsal root rhizotomy are differentand may indicate thedifferentroles of BDNF and NT-3 in lamina II afterspinal cordinjury. 〔

关 键 词：脑源性神经营养因子 神经营养因子-3 免疫组织化学 Ⅱ板层 背根切断术 

分 类 号：R651[医药卫生—外科学]