雄性小鼠联会复合体形成过程的电镜观察  

Electron microscopic observation on process of synaptonemal complex forming in male mice

作　　者：牛新华[1] 谢英勃[2] 李鸿业[1] 

机构地区：[1]滨州医学院生物学教研室,滨州256603 [2]山东师范大学电镜室

出　　处：《解剖学杂志》2002年第5期484-488,共5页Chinese Journal of Anatomy

摘　　要：目的 :通过观察小鼠精母细胞联会复合体形成过程 ,研究减数分裂时同源染色体的配对行为。方法 :用本室改进的微铺展 硝酸银染色技术制备小鼠精母细胞联会复合体样品 ,进行电镜观察。结果 :同源染色体配对时 ,大多数是两个端粒开始接触 ,联会复合体似拉链一样扩展。有的在同源染色体内部区域的一点或多点同时开始配对 ,联会以扣纽扣方式进行。XY染色体配对仅限于部分区域。一个细胞内不同对染色体的联会不同步。同源染色体交叉在联会过程中形成 ,大多数同源染色体形成一个或多个交叉 ,但有的一个交叉也没有。结论 :大多数同源染色体以不同方式进行配对 ,交叉互换的数目也各不相同。Objective:To study on action of homologues pairing during miosis and to observe the process of synaptonemal complex (SC) forming in mice spermatocytes.Methods:With improved microspreading and silver staining technique,SCs samples were observed by transmission electron microscope.Results:During homologues pairing 2 telomeres of most homologues started touch and SCs extended as zipper.Some homologues pairing was in progress at the same time on one or more points inside them.Synapsis was in progress as button.Pairing between X and Y chromosomes was confined to part of them.Synapses of different pairs of chromosomes in one cell didn't synchronize.Chiasmata of homolgues formed in the process of synapsis.In most homolgues one or more chiasmata formed,but in some homologues no chiasma were formed.Conclusion:Homologues pairing in mice is in different ways,the number of chiasmata and crossing over is various too.

关 键 词：雄性小鼠 联会复合体 电镜观察 同源染色体 

分 类 号：Q953[生物学—动物学]