SZ-1对脑血管内皮及平滑肌细胞释放花生四烯酸的影响  

Inhibitory Effect of SZ-1 on the Arachidonic Acid Releases in the Cerebrovascular Endothelial and Smooth Muscle Cells

作　　者：孙笃新[1] 黄彤舸[1] 曾国钱 朱驹 芮耀诚 

机构地区：[1]第二军医大学药学院药理学教研室,200433

出　　处：《第二军医大学学报》1992年第4期312-317,共6页Academic Journal of Second Military Medical University

摘　　要：在体外培养的脑微血管内皮细胞及大脑前动脉平滑肌细胞上研究了血小板激活因子(PAF)对^(14)C-花生四烯酸(^(14)C-AA)释放的作用,并观察了新合成药物SZ-1的作用。结果表明^(14)C-AA在此两种细胞上参入很快,4h可达平衡,PAF 0.1～20μmol/L能显著刺激AA释放。SZ-1 0.2～20μmol/L能剂量依赖性地抑制PAF诱导的AA释放,且可剂量依赖性地抑制PAF诱导的兔洗涤血小板的聚集,但对由AA,ADP诱导的PRP的聚集无抑制作用,亦不能抑制脑微血管内皮细胞产生PAF,结果提示,SZ-1具有特异性的PAF受体拮抗作用。The release of platelet activating factor (PAF) induced 14C-arachidonic acid (14C-AA) in the bovine cerebral microvascular endothdial cells (CMEC) and arterior cerebral artery smooth muscle cells (ACASMC) and the antagonism of SZ-1 are described. The results showed that 14C-AA incorporated into the cells rapidly and PAF 0.1-20μmol/L dose-dependently stimulated the AA release significantly. It indicated that the action of PAF on the cerebrovascular system was associated with the stimulation of AA release. SZ-1 0.2-20μnol/L dose-dependently inhibited the PAF induced AA release in CMBC and ACASMC, and PAF induced aggregation of washed rabbit platelets, but did not inhibited ADP or AA induced aggregation of platelet-rich plasma(PRP), and PAF production in CMEC, indicating the specific antagonism of SZ-1 on PAF receptor.

关 键 词：脑动脉 血管内皮 花生四烯酸 牛 

分 类 号：R743.02[医药卫生—神经病学与精神病学]