抗血管内皮生长因子抗血清对小鼠膀胱癌的影响  

The effect of anti-VEGF antiserum on the progression of bladder cancer in mouse model

作　　者：王嵩[1] 张智清[2] 刘漓波[1] 曾荔[1] 薛兆英[1] 那彦群[1] 郭应禄[1] 

机构地区：[1]北京大学第一医院,北京大学泌尿外科研究所100034 [2]中国预防医学科学院病毒基因工程国家重点实验室

出　　处：《中华泌尿外科杂志》2002年第12期742-744,共3页Chinese Journal of Urology

摘　　要：目的　观察抗血管内皮生长因子 (VEGF)抗血清对小鼠膀胱肿瘤血管生成和肿瘤生长的影响 ,探讨膀胱肿瘤治疗的新途径。　方法　采用重组VEGF蛋白制备抗VEGF抗血清 ,ELISA方法检测其特异性 ;建立小鼠膀胱癌模型 ,并将荷瘤小鼠随机分为抗VEGF抗血清组和对照组 ,分别经腹腔注射抗VEGF抗血清和普通小鼠血清 ;检测肿瘤内微血管密度 (MVD) ,并称取肿瘤重量。　结果　实验制备了高度特异性的抗VEGF抗血清 ,荷瘤小鼠用药后抗VEGF抗血清组MVD值 (7.5 1± 2 .88)明显低于对照组 (14 .4 8± 3.92 ) ,抗VEGF抗血清组小鼠平均瘤重较对照组稍轻 ,P =0 .6 0 5 ,差别无显著性意义。　结论　抑制VEGF作用可以减少膀胱肿瘤的血管生成 ,为膀胱肿瘤抗血管生成疗法提供了依据 ,并可能成为膀胱肿瘤重要的辅助治疗手段之一。Objective To investigate the effect of antiserum against VEGF on angiogenesis and growth of bladder cancer in a mouse model in order to explore a novel strategy for the treatment of bladder cancer. Methods Antiserum against VEGF was prepared by using recombinant human VEGF and was identified for its specific affinity with VEGF by ELISA.The bladder carcinoma cell lines BST739 were implanted in T739 mice which were randomly divided into 2 groups:the control group receiving normal mice serum intraperitoneally,and the experimental group receiving antiserum against VEGF intraperitoneally.All the animals were sacrificed at last.Then all the bladder carcinomas were weighted and the microvessel density (MVD) in them was studied by immunohistochemical staining. Results The antiserum against VEGF was prepared and demonstrated with high specificity. The MVD in the antiserum group was lower than that in the control group. But the average weight of tumors was not significantly less in the antiserum group. Conclusions The study shows that to inhibit the activity of VEGF may reduce the angiogenesis in bladder cancer and supports the antiangiogenic therapy for bladder cancer.Inhibition of VEGF activity might become an important way of adjuvant therapy for bladder cancer.

关 键 词：抗血管内皮生长因子 抗血清 小鼠 膀胱癌 

分 类 号：R737.14[医药卫生—肿瘤]