癫痫病人血细胞多药耐受基因表达产物细胞膜糖蛋白的检测  

Detecting overexpression of P-glycoprotein-multidrug resistance gene in epileptic patient's peripheral blood lymphocytes

作　　者：王学峰[1] 吕洋[1] 黄蕾[1] 文世全[1] 晏勇[1] 

机构地区：[1]重庆医科大学附属第一医院神经内科,400016

出　　处：《中华神经科杂志》2002年第6期348-350,共3页Chinese Journal of Neurology

基　　金：卫生部科研基金资助项目 ( 98 2 0 0 1)

摘　　要：目的　探索血白细胞多药耐受 (MDR)基因表达与难治性癫痫耐药性的关系。方法　用免疫组化和流式细胞仪两种方法检测癫痫病人血中MDR基因的表达产物细胞膜糖蛋白 ,并对其结果进行临床验证。结果　流式细胞仪检测 49例 ,其中 1 1例表达阳性的患者对随后进行的治疗无效 ,而表达阴性的 32例患者中 1 8例有效 ;免疫组化检测 34例 ,2 2例阳性患者中仅 2例有效 ,1 2例表达阴性的患者中 7例有效。结论　血中MDR的表达可作为难治性癫痫病人耐药性的重要参考指标 ,流式细胞仪检测的敏感性较免疫组化差 。Objective To explore the relationship between the overexpression of P glycoprotein (P GP) multidrug resistance (MDR) gene in the epileptic patient's peripheral blood lymphocytes and the drug resistance of the intractable epilepsy Methods This is a prospective, observational study. First, the overexpression of P GP MDR in 85 epileptic patients' (M 39, F46,overage age 24 years) peripheral blood lymphocytes were investigated by using immunocytochemistry (ICT) or flow cytometer (FCE). Then, patients were given a single or a combining of two antiepilepstic drugs which they did not received before (including carbamazepine, valproic acid, phenobarbitone,phenytoin), when the single drug was not effective in observing the clinical efficacy Results In FCE study group, results showed that the 11 patients with overexpression of P GP MDR in the epileptic patient's peripheral blood lymphocytes became tolerant to antiepileptic drugs. Eighteen out of 32 patients without overexpression of P GP MDR are effective. In the ICT study group, it is effective that there are only 2 out of the 22 patients with overexpression of P GP MDR. Seven of 12 patients without overexpression of P GP MDR are effective Conclusion It is suggested that the overexpression of MDR in the intractable epileptic patient's peripheral blood lymphocytes might be a significant drug resistance marker. The sensibility of ICT method should be better than the FCE,though the latter may be more accurate.

关 键 词：癫痫 血细胞 多药耐受 基因表达 细胞膜糖蛋白 检测 免疫组织化学 流式细胞术 

分 类 号：R742.1[医药卫生—神经病学与精神病学]