E-Cadherin和CD44v6对Tca8113细胞系侵袭性影响的实验研究  

E-cadherin and CD44v6 Influencing the Invasion of Oral Squamous Cell Carcinma Cell Line in vitro

作　　者：田臻[1] 郭伟[2] 张伟国[1] 周晓健[2] 

机构地区：[1]上海第二医科大学附属第九人民医院口腔医学院口腔病理科,上海200011 [2]上海第二医科大学附属第九人民医院口腔医学院口腔颌面外科,上海200011

出　　处：《上海口腔医学》2002年第4期356-359,共4页Shanghai Journal of Stomatology

基　　金：卫生部科学研究基金(96-2-293);国家教委骨干教师资助计划的资助(GGJS200004)

摘　　要：目的通过检测粘附分子在立体胶原凝胶培养系统中对口腔黏膜鳞状细胞癌(SCC)细胞侵袭性的影响,加深了解口腔癌细胞侵袭和转移的可能机制,并评估其作为口腔癌预后判断指标的可行性。方法应用免疫组织化学方法检测粘附分子E-cadherin(E-cad)和CD44v6在舌鳞癌细胞系Tca8113细胞的表达;同时在体外建立鼠尾胶原凝胶三维立体侵袭系统,在此系统中观察粘附分子对Tca8113细胞侵袭胶原能力的影响。结果免疫组织化学检测显示,E-cad在Tca8113细胞有阳性表达,而CD44v6表达阴性;胶原凝胶侵袭实验表明:E-cad可以有效地减少Tca8113细胞在胶原凝胶中的浸润细胞数和程度(0.01<P<0.05)。结论虽然粘附分子只是影响肿瘤侵袭和转移的重要因素之一,但体外实验表明:E-cad具有抑制肿瘤细胞胶原侵袭的作用,提示E-cad有可能成为一种较好的临床评判口腔黏膜鳞癌预后的有用指标。Objective To observe if adhesion molecules can affect the invasion behavior of squamous cell carcinoma cell line in 3-Dimension collagen gel in vitro. It will provide the experimental basis for clinic application of adhesion molecules as marker to evaluate the prognosis of oral cancer patients, and effectively improve the survival rate of oral cancer patients in future. Methods Immumohistochemistry method was used to detect the expression of adhesion molecules E-cadherin(E-cad) and CD44v6 in squamous cell carcinma cell line(Tca8113), as well as to build a 3-dimension collagen gel system in vitro. In this system the effect of invasion behavior of Tca8113 cells was observed using antibodies of adhesion molecules as a blocker. Results Significant expression of E-cad in the Tca8113 cells was observed, but that of CD44v6 was insignificant. 3-dimension collagen gel assay in vitro revealed that E-cad antibodies could effectively decrease the number of invaded cells and the degree of Tca8113 cells migrated into gel (P=0.013,0.01<P<0.05). Conclusions E-cad can suppress the invasion of squamous cell carcinoma cells into collagen gel in vitro. It indicates that E-cad may be a helpful marker to evaluate the prognosis of oral squamous cell carcinoma.

关 键 词：E-CADHERIN CD44V6 TCA8113 细胞系 口腔粘膜鳞状细胞癌 肿瘤侵袭 肿瘤转移 

分 类 号：R780.2[医药卫生—口腔医学]