精神分裂症患者BDV-p24基因的扩增及其产物的测序鉴定  

Amplication and Seqnence Confirmation of Borna Disease Virus p24 Gene among Schizophrenic Patients

作　　者：李桂梅[1] 张凤民[1] 马培林[1] 杨爱英[1] 谷鸿喜[1] 温捍淑 刘雪峰 生田和良[4] 

机构地区：[1]哈尔滨医科大学微生物学教研室,150086 [2]哈尔滨市精神病院 [3]大庆市油田总医院 [4]日本大阪大学微生物病研究所

出　　处：《神经疾病与精神卫生》2002年第6期321-322,共2页Journal of Neuroscience and Mental Health

基　　金：国家自然科学基金(批准号:30070673);哈尔滨市科委后备带头人基金(批准号:7271);黑龙江省教育厅骨干教师基金

摘　　要：目的 扩增精神分裂症患者PBMCs标本中博尔纳病病毒(Borna Disease Virus,BDV)p24基因,并对基因扩增产物进行测序鉴定,分析其与标准株之间的差异。方法 用巢式RT—PCR方检测黑龙江省精神分裂症患者及正常人PBMCs中BDV-p24基因片段,对2例BDV—p24基因阳性的巢式RT-PCR产物进行测序,并与标准株比较。结果 9例精神分裂症患者中有2例BDV-p24基因阳性,7例正常人标本中未发现BDV—p24基因阳性。测序结果进一步证实扩增产物为BDV—p24基因,其序列与标准株高度同源。结论 用巢式RT—PCR方法可以特异性扩增出BDV—p24基因,扩增产物序列与标准株高度同源,提示黑龙江省的精神分裂症的发生可能与BDV感染有关。To amplify the BDV p24 gene among schizophrenic patients and investigate the sequence discrepancies of BDV - p24 gene. Methods Nested RT - PCR was used to detect BDV - p24 gene from PBMCs of schizophrenic patients and normal controls of HeiLongJiang province, two positive nested -PCR products were sequenced and compared with the standard strains. Results 2 of 9 schizophrenic patients were found to be BDV- p24 positive, while no positive result was detected among normal controls. BDV -p24 sequences showed high conservation with standard strains. Conclusions BDV p24 gene could be amplified specifically by use of RT - PCR, BDV infections probably exist in schizophrenic patients of HeiLongJiang province.

关 键 词：精神分裂症 BDV-p24基因 产物 测序 鉴定 基因扩增 

分 类 号：R749.3[医药卫生—神经病学与精神病学]