日达仙体外诱生免疫相关细胞因子水平的实验研究  

EXPREIMANTAL STUDY ON THE LEVEL OF THE CYTOKINS KELATED IMMUITY INDUCED BY ZADAXIN IN PBMNC

作　　者：张正文[1] 李晓诗[1] 金逸[1] 王静月[2] 

机构地区：[1]江苏省常熟市第一人民医院,江苏常熟215500 [2]苏州大学核医学生物技术重点实验室,江苏苏州215007

出　　处：《肿瘤研究与临床》2002年第6期369-371,共3页Cancer Research and Clinic

摘　　要：目的 :研究日达仙体外诱导 PBMNC中 T淋巴细胞及其亚群和 NK细胞水平以及免疫相关细胞因子的作用。方法 :应用流式细胞仪检测 PBMNC中 T淋巴细胞及其亚群和 NK细胞水平 ;EL ASA酶联免疫法分析细胞因子 (IFNγ、TNFα、IL- 2、IL- 6、IL- 8、IL- 12和 IL- 18)的水平。结果 :PBMNC在不同的培养条件下 ,各组间 T淋巴细胞及其亚群和 NK细胞水平无显著性差异 (P>0 .0 5 )。日达仙组培养上清中 IL- 2、IL- 8和 IFNγ水平高于对照组 ,差异有显著性 (P<0 .0 5 )。日达仙加 PHA组的 IL- 2、IL- 12水平高于 PHA组 ,差异有显著性 (P<0 .0 5 ) ;加 SMMC组的 IL- 2、IL- 12和TNFα水平亦高于 SMMC- 772 1组 ,差异有显著性 (P<0 .0 5 )。结论 :日达仙能显著地促进 PBMNC合成与分泌多种免疫相关细胞分子。Objective:To investigate the level of cytokins related immunity.T lymphocyte cells and subsets was induced by Zadaxin in peripheral blood mononuclear cells (PBMNC).Methods:The level of T lymphocyte cells and subsets,NK cells was observed by Flow cytometry that it was in different culture conditions.The level of cytokins related immunity (IL 2,IL 6,IL 8,IL 12,IL 18,TNFα and IFNγ) was observed by ELASA.Results:The level of T lymphocyte cells and subsets,NK cells in PBMNC in different culture conditions,no significant difference was observed ( P >0.05).The level of IL 2,IL 8 and IFNγ in Zadaxin culture supernatant increased evidently compared with the contrast PHA group ( P <0.05).Conclusion:Zadaxin can obviously boost the compose and secrete of the many cytokins related immunity in PBMNC.

关 键 词：日达仙 免疫相关细胞因子 实验研究 细胞培养 抗肿瘤作用 

分 类 号：R73-36[医药卫生—肿瘤]