GDN法及其在原生动物棘尾虫研究中的应用  

GDN method and its application on the study of the protozoan stylonychia mytilus

作　　者：舒斯云[1] 包新民[1] 陈长才[1] 张小云[2] 卢丽[2] 

机构地区：[1]第一军医大学珠江医院神经生物研究室 [2]深圳大学生命科学研究室

出　　处：《第一军医大学学报》1992年第4期285-288,共4页Journal of First Military Medical University

摘　　要：本研究应用一种敏感的硫酸镍胺增强葡萄糖氧化酶—二氨基联苯胺呈色法(GDN法),首次在单细胞原生动物棘尾虫体内发现P物质、脑腓肽、生长抑素、胆囊收缩素、神经降压肽及神经肽Y等6种神经肽和酪氨酸羟化酶以及FOS蛋白的定位表达。对GDN法的原理、优点,应用前景及在原生动物棘尾虫首次发现神经肽等活性物质的意义进行了讨论。Using a very sensitive immunohistochemistry method, the nickel ammonium sulfate and glucose oxidase enhacing diaminobenzidine ( GDN ) technique, the expression and localization of neuropeptide Y ( NPY ) , cholecystokinin ( CCK ) , somatostatin ( SOM ) , neurotensin ( NT ) , leu-enkephalin ( L-ENK ) , substance P ( SP ) , tyrosine hydroxylase ( TH ) , and FOS were demonstrated in the protozoan, STYLONYCHIA MYTILUS. The principle and advantages of the GDN method and the meaning of the expression of neuropeptides and FOS were discussed.

关 键 词：硫酸镍胺 GDN法 神经肽 棘尾虫 

分 类 号：R338[医药卫生—人体生理学]