成团肠杆菌几丁质酶活性和抑真菌活性的遗传调控  

Genetic regulation of chitinolytic and antifungal activity in Enterobacter agglomerans

作　　者：周乐聪[1] Leonid S.Chernin 

机构地区：[1]中国农业科学院油料作物研究所,农业部油料作物遗传改良重点实验室,国家油料作物改良中心,湖北武汉430062 [2]以色列耶路撒冷希伯莱大学农学院,rehovot76100

出　　处：《中国油料作物学报》2002年第4期50-53,共4页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(39970502);中以农业研究基金(SIARF2001-03);人事部留学回国人员科技活动择优资助项目

摘　　要：通过3亲本交配,将供体菌株携带外源调节基因的质粒转移到植病生防细菌成团肠杆菌(E.agglomerans)IC1270中,得到4个衍生菌株。几丁质酶活性测定表明,衍生菌株对几丁质的消解能力均显著高于亲本菌株,以携带gacA或rpoD衍生菌株的活性最高。离体抗真菌颉抗实验表明,携带gacA或rpoD衍生菌株比亲本菌株对核盘菌和立枯丝核菌的抑制效果显著增强,其它衍生菌株无明显提高。Four E.agglomerans IC1270 derivatives carrying heterologous regulatory gene (gacA, rpoD, rpoS, or apdA) from Pseudomonas fluorescens were constructed by triparental mating. Measuring chitinase activity showed that the chitinolytic activity of all the four derivatives was enhanced in comparison with their parental strain. The in vitro antifungal activity of the derivatives carrying gacA or rpoD was also markedly increased when tested with Sclerotinia sclerotiorum or Rhizoctonia solani as targets. The results suggested heterologous regulatory genes gacA and rpoD possess strong positive regulatory effect on elevating the biocontrol potential of E.agglomerans IC1270.

关 键 词：成团肠杆菌 几丁质酶 抑真菌活性 遗传调控 调节基因 拮抗作用 酶活性 

分 类 号：Q344.3[生物学—遗传学]