ICA,PJM逆转肿瘤细胞恶性表型及其机制的研究  

The Reversion of Tumor Cells Malignant Phenotype Treated by ICA and PJM and Its Mechanism

作　　者：薛兴奎[1] 张玲[1] 王芸[1] 毛海婷[1] 李登华[1] 温培娥[1] 崔树龄[1] 

机构地区：[1]山东省医学科学院基础医学研究所,济南250062

出　　处：《中国肿瘤生物治疗杂志》2002年第4期272-275,共4页Chinese Journal of Cancer Biotherapy

基　　金：山东省科技厅资助项目(971226206)

摘　　要：目的:研究ICA,PJM逆转肿瘤细胞恶性表型,提高免疫效应细胞识别和杀伤的作用和机制。方法:MTT法检测ICA,PJM对PG细胞增殖的影响以及对CD3AK杀伤敏感性的影响;RT-PCR法检测ICA,PJM对bcl-2和c-fos基因mRNA表达水平的影响;流式细胞术检测细胞表面HLA-A,B,C抗原表达的变化以及c-fos,bcl-2蛋白表达水平的变化。结果:ICA,PJM对PG细胞有明显的增殖抑制作用,可以提高细胞表面HLA-A,B,C分子的表达和c-fos蛋白的表达,抑制bcl-2基因的表达,提高CD3AK细胞对PG细胞的杀伤活性。结论:ICA,PJM可以逆转肿瘤恶性表型,提高免疫效应细胞对肿瘤细胞的识别和杀伤。Objective:To explore the mechanism and reversion of malignant phenotypes in a highly metastatic human lung tumor cell line. Methods: Suppression of 1C A and PJM on PG cell line and the effects of PJM and 1C A on PG cells sensitivity to lysis by CD3AK effectors cell was studied by MTT assay; the expression of bcl-2 and c-fos was studied by RT-PCR and flow cytometry, the expression of HLA-A, B, C in PG cells was examined by flow cytometry. Results:ICA and PJM inhibited the growth of PG obviously. ICA and PJM could significantly enhance the expression of HLA-ABC antigen and c-fos gene. ICA and PJM decreased the expression of bcl-2 and enhanced the the susceptibility of PG cells to lysis by CD3AK cells. Conclusion:ICA and PJM can reverse the malignant phenotypes of PG cells, enhance the recognition and killing effect of T cells.

关 键 词：肿瘤细胞 PJM ICA HLA-AB C抗原 bcl-2 c-fos 免疫治疗 肺巨细胞腺癌 

分 类 号：R730.51[医药卫生—肿瘤] R734[医药卫生—临床医学]