卡那霉素抗性基因在三孢布拉霉菌种中的表达  

Expression of kanamycin resistance in Blakeslea trispora

作　　者：单志萍[1] 陆茂林[1] 孟妤[1] 胡幼敏[1] 

机构地区：[1]江苏省微生物研究所,无锡214063

出　　处：《工业微生物》2002年第4期24-27,共4页Industrial Microbiology

基　　金：江苏省自然基金资助项目 ;(编号BK9716 7)

摘　　要：利用一个带有自主复制子 ,但缺失自身启动子的源于转座子Tn - 5的卡那霉素抗性基因的质粒PVB32 ,在大肠杆菌中克隆丝状真菌三孢布拉霉DNA中有启动子功能的DNA片段 ;通过原生质体转化 ,获得了三孢布拉霉对卡那霉素抗性的表达 ,且抗性表现稳定 ,可通过孢子无性繁殖稳定遗传下去。Using a plasmid which carrying the kanamycin resistance gene but lacking its own promoter, the DNA fragment having promoter activity of Blakeslea trispora was successfully cloned in Escherichia coli DH1. The sequences of cloned DNA fragmentwere effective in promoting resistance to kanamycin when the corresponding plasmids were introduced in the Blakeslea trispora via protoplast transformation. The resistance phenotype was stable and could be propagated vegetatively via spores.

关 键 词：卡那霉素抗性基因 三孢布拉霉 菌种 表达 真菌转化 Β-胡萝卜素 

分 类 号：Q933[生物学—微生物学]