芽孢杆菌发酵条件的优化及其在室内条件下提高原油采收率的初步研究  

Optimization of conditions for fermentation By Bacillus L-23 and preliminary study on utilization of L-23 for enhancing oil recovery

作　　者：李清心[1] 康从宝[1] 王浩[1] 张长铠[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,济南250100

出　　处：《工业微生物》2002年第4期28-31,共4页Industrial Microbiology

摘　　要：筛选到一株可以在厌氧条件下以原油为唯一碳源生长的芽孢杆菌L - 2 3。对其中试发酵条件进行了优化 ,确定了L - 2 3的最适发酵条件 ,用补料的方法控制葡萄糖浓度在 0 .6% ,在 16h内 ,发酵液的菌体浓度可以达到 5 .2× 10 9cell mL。把 5 %的L - 2 3发酵液加入到原油中 ,在 4 5℃条件下培养 2 4h ,能够使原油粘度降低 2 8%。在室内条件下 ,L - 2 3可以使原油的采收率提高 11%以上。实验证明 ,用L - 2 3进行微生物采油有很好的应用前景。The Bacillus L-23 using crude oil as the sole carbon source under anaerobic conditions was screened. The fermentation condition of L-23 and its cultural conditions were optimized. The density of the L-23 could reached 5.2×109 cell/mL in 16 hours when the concentration of glucose was kept being 0.6% by feeding nutrient supplement. The results showed that L-23 could reduce crude oil viscosity by 28% when 5% of L-23 was added to the crude oil and cultivated at 45℃ for about 24 hours. In the laboratory, L-23 could enhance oil recovery by more than 11%. The experiment demonstrated that L-23 had great potential in the microbial enhanced oil recovery.

关 键 词：芽孢杆菌 发酵条件 优化 室内条件 原油采收率 原油降粘 

分 类 号：TE357[石油与天然气工程—油气田开发工程]