β-葡萄糖苷酶产生菌的分离筛选  

Isolation and screening of β-glucosidase producing strain

作　　者：杨胜远[1] 刘玉燕[1] 梁智群[1] 

机构地区：[1]广西大学食品发酵工程研究所,南宁530004

出　　处：《工业微生物》2002年第4期36-38,共3页Industrial Microbiology

基　　金：国家自然科学基金 ( 2 0 0 6 6 0 0 1);广西大学科研基金资助项目

摘　　要：从土样中分离筛选到 3株 β -葡萄糖苷酶产生菌 ,其中As .n .XD - 1酶活力最高 ,pNPG酶活为 19.67U ,纤维二糖酶活为 31.4 7U。该 β -葡萄糖苷酶在pH4～ 6及 4～ 60℃之间较稳定 ;4℃存放 60d酶活仍可保留 90 .6%。粗酶液中还含有α -葡萄糖苷酶 (0 .0 2U)、淀粉酶 (1.13U)、纤维素酶 (0 .16U)和CMC酶 (1.18U)。From the soil samples, three β-glucosidase producing strains were isolated and screened, in which the As.n.XD-1 strain was the highest producer. The pNPG activity reached 19.67U and the cellobiose activity reached 31.47U.It was stable at pH4～6 and 4～60℃. After reserved at 4℃ for 60d, the β-glucosidase activity could retain 90.6%. Besides β-glucosidase, the crude enzyme succus also contained some other enzymes included α-glucosidase(0.02U), amylase(1.13U), cellulase (0.16U)and CM-cellulase(1.18U).

关 键 词：β-葡萄糖苷酶产生菌 分离筛选 酶活力 黑曲霉 

分 类 号：Q939.9[生物学—微生物学]