利用套叠PCR技术改造酵母表达载体pAO815的研究  

Reconstruction of pAO815 of Yeast Expression Vector by Overlap Extension

作　　者：周鹏[1] 郭安平[1] 黎小瑛[1] 

机构地区：[1]中国热带农业科学院热带作物生物技术国家重点实验室,中国海南海口571101

出　　处：《生命科学研究》2002年第4期310-313,共4页Life Science Research

基　　金：国家自然科学基金资助项目(30060033)

摘　　要：利用套叠PCR技术将α factor信号肽基因插入pAO815之EcoRⅠ位点,构建成分泌型表达载体pAO815α A.在不改变原克隆位点EcoRⅠ的条件下,借助pAO815之第873位的HindⅢ位点,将pAO815AOX1启动子873 940片段连同α factor信号肽基因序列(246bp)插入pAO815之HindⅢ EcoRⅠ之间,构建成含α factor信号肽基因的分泌型表达载体pAO815α A.The pAO815αA of secrete expression vector was constructed with αfactor signal peptide gene inserted into EcoR Ⅰ site of pAO815 by overlap extension of PCR technique,which 873th940th bps fragment of pAO815 AOX1 promoter with αfactor signal peptide gene was inserted into Hind Ⅲ/EcoR Ⅰ site and a kind of pAO815 containing αfactor signal peptide gene was constructed under nonchanging previous EcoR Ⅰ cloning site.

关 键 词：套叠PCR技术 改造 酵母表达载体 pAO815 分泌型表达载体 

分 类 号：Q782[生物学—分子生物学]