特异性神经元抗Hu抗体对小细胞肺癌早期诊断价值的研究  

The value of specific neuronal anti-Hu antibody in the early diagnosis of small cell lung cancer

作　　者：王刚[1] 陈齐鸣[2] 祝延[1] 柯道平[1] 钱伟东[2] 宋文英[2] 

机构地区：[1]安徽医科大学生理学教研室神经生理实验室,合肥230032 [2]蚌埠医学院附属医院神经内科

出　　处：《中华结核和呼吸杂志》2002年第12期732-735,共4页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的　探讨特异性神经元抗Hu抗体对小细胞肺癌 (SCLC)早期诊断的价值。方法　采用亲合免疫细胞组织化学卵白素 生物素过氧化物酶复合物法 (ABC法 )、蛋白免疫印迹法 (Westernblot)对 98例SCLC患者的血清进行检测 ,分析其临床意义。结果　 (1)亲合免疫细胞组织化学ABC法在约 40 %不伴神经系统副肿瘤综合征 (PNSNS)的SCLC患者血清中检测出特异性神经元抗体 ,滴度为1∶10 0 0～ 1∶80 0 0 ;在约 89%伴PNSNS的SCLC患者血清中检测出特异性神经元抗体 ,滴度为 1∶10 0 0～1∶64 0 0 0 ;(2 )Westernblot在不伴PNSNS的SCLC患者血清中检测出 2例可识别正常人大脑神经元细胞核提取物中分子量为 3 5 0 0 0～ 40 0 0 0蛋白和 40 0 0 0的HuD克隆纯化蛋白抗原 ;在伴PNSNS的SCLC患者血清中检测出 16例可识别上述蛋白抗原 ;(3 )正常人血清中未检测出特异性神经元抗体。结论　特异性神经元抗Hu抗体对SCLC的早期诊断具有重要意义 。Objective To evaluate the value of specific neuronal anti Hu antibody in the early diagnosis of small cell lung cancer (SCLC) Methods Ninety eight patients with SCLC were included in the study Specific neuronal anti Hu antibody was detected by immunohistochemistry (the ABC method) and Western blot Results The sera from 40%(32/80) of the patients in group A ( without paraneoplastic syndrome of the nervous system) reacted with the frozen sections of human cerebra and were stained by indirect immunoperoxidase method at final dilutions of 1∶1 000 to 1∶8 000, while the sera from 89% of the patients (16/18) in group B (with paraneoplastic syndrome of the nervous system) reacted at final dilutions of 1∶1 000 to 1∶64 000 Western blot analysis revealed that the specific neuronal anti Hu antibody was identified at several bands of approximately 35 000～40 000 withnuclear extracts, and at 40 000 with HuD clonal protein The anti Hu antibody was detected in only two patients from group A, but in 16 patients from group B The antibody was not detected in sera from 32 normal human subjects Conclusions Specific neuronal anti Hu antibody at high titers was detected in patients with SCLC not complicated with paraneoplastic syndrome of the nervous system.This antibody may be useful in the early diagnosis of SCLC

关 键 词：特异性神经元抗Hu抗体 小细胞肺癌 神经系统副肿瘤综合征 诊断 

分 类 号：R734.2[医药卫生—肿瘤]