红景天对大鼠血清和组织匀浆中肝纤维化相关酶活性的影响  

THE EFFECTS OF RHODIOLA SACHALINENSIS A BOR ON THE ACTIVITES OF FIBROSIS-ASSOCIATED ENZYMES IN SERUM AND TISSUE IN RATS OF CCL_4-INDUCED LIVER FIBROSIS

作　　者：吴晓玲[1] 曾维政[2] 陈晓斌[2] 蒋明德[2] 熊碧君[2] 张勇[2] 徐辉[2] 

机构地区：[1]第三军医大学研究生大队,重庆400038 [2]成都军区总医院消化内科,四川成都610083

出　　处：《华西药学杂志》2002年第6期416-418,共3页West China Journal of Pharmaceutical Sciences

基　　金：成都军区医药卫生科研基金资助课题 (编号 0 1Y0 1)

摘　　要：目的　观察红景天干预性治疗对大鼠血清与肝组织匀浆中 β -NAG、BDGP、MAO、ALD 4种肝纤维化相关酶活性的影响 ,探讨组织和血清中肝纤维化相关酶活性变化的关系及其与肝组织病理学变化的相关性。方法　SD大鼠随机分 3组 ,A组用四氯化碳皮下注射法诱导大鼠肝纤维化模型 ,B组造模同时给予红景天粉剂口服 ,C组为正常对照组。造模结束检测大鼠血清及肝组织匀浆中 4种肝纤维化相关酶活性的变化以及肝脏组织病理学的改变 ,并进行相关性分析。结果　口服红景天可抑制大鼠血清及肝组织匀浆 β -NAG、BDGP、MAO、ALD的活性 ,明显改善大鼠肝组织病理学变化 ,其组织匀浆酶活性、血清酶活性、肝组织病理变化三者呈正相关。结论　红景天可明显降低组织及血清肝纤维化酶谱活性 ,改善肝组织病理学变化 。OBJECTIVE To study the effects of Rhodiola sachalinensis A Bor on activities of N-acetyl-beta-D-glucosaminidase(β-NAG),beta-D-glucopyranosidase(BDGP), monoamine oxidase(MAO) and aldolase(ALD) in serum and tissues in rats of CCl 4 -induced liver fibrosis, and compare the levels of these enzymes with liver histopatholological changes.METHODS Male SD rats were given 30% CCl 4(3 ml·kg -1 ,2 times·week -1 ) for 15 weeks. Rhodiola sachalinensis A Bor was fed to the treatment group (0.5 g·kg -1 ) during the modelling time. The activities of serum and tissue fibrosis-associated enzymes were detected with spectrophotometric method and histopatholological changes with HE,VG,Masson staining.RESULTS In comparison with normal controls, the activities of the β-NAG , BDGP, MAO, and ALD in serum and tissue of the model rats were significantly increased( P <0.05) Rhodiola sachalinensis A Bor treatments decreased the degree of the elevation and the changes correlated to the histopathological improvement.CONCLUSION Rhodiola sachalinensis A Bor is effective in decreasing the activities of serum and tissue β-NAG , BDGP, MAO, and ALD. It can improve the histopathological changes in the liver,thus protect CCl 4 induced liver fibrosis in rats.

关 键 词：红景天 肝纤维化 大鼠 β-NAG BDGP MAO ALD 中药 药理 

分 类 号：R575.205[医药卫生—消化系统] R28[医药卫生—内科学]