牛磺酸在大鼠缺血再灌注过程中对肝线粒体的保护作用  

The Protective Effect of Taurine on Rat Liver Mitochondria after Ischemia and Reperfusion

作　　者：汪朝晖[1] 张贤康[2] 缪明永[3] 王学敏[3] 

机构地区：[1]中国人民解放军第81医院肝病研究所,南京210002 [2]第二军医大学附属长征医院 [3]第二军医大学

出　　处：《中西医结合肝病杂志》2002年第6期351-354,357,共5页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:观察缺血再灌注过程中线粒体的变化和牛磺酸的保护作用。方法:实验动物分为3组:假处理组(对照组),阻断组,阻断+牛磺酸组(保护组)。线粒体分离后测定呼吸功能、琥珀酸氧化酶、NADH氧化酶、细胞色素C氧化酶活性、MDA、GSH含量、钙、镁含量和游离钙的浓度。结果:肝缺血再灌注以后,线粒体呼吸功能和氧化磷酸化功能显著降低,引起线粒体脂质过氧化损伤,并可造成线粒体内GSH下降,使线粒体抗氧化系统活性下降;同时可引起线粒体内钙积聚、游离钙浓度明显升高以及镁的丢失。这些结果说明线粒体能量代谢障碍是缺血再灌注损伤的始动环节,而氧自由基是造成损伤的主要因素。牛磺酸对缺血再灌注损伤过程中线粒体损伤的各个环节均起保护作用。结论;研究表明牛磺酸作为自由基清除剂在体内可能具有非常重要的生理学作用。Objective: To observe the change of rat liver mitochondria after ischemia-reperfusion and the protective effect of taurine. Methods: All rats were divided into three groups: sham operation, ischemia-reperfusion untreated and ischemia-reperfusion taurine treated. Hepatic mitochondria were isolated, mitochondrial respiratory function, the activity of Suc-oxi-dase, NADH-oxidase, Cytc-oxidase, the content of MDA, GSH, Calcium, Magnesium and free ionized calcium concentration were measured. Results: Mitochondrial respiratory function was significantly declined and increased lipid peroxide contents were observed after ischemia-reperfusion, the fact that GSH in mitochoudria decreased indicated that the activity of antioxida-tion system decreased. Accumulation of calcium in mitochondria, free ionized calcium concentration increased markedly and mitochondrial magnesiumcontents reduced dramatically in ischemia-reperfusion. These results showed that the energy metabolism dysfunction was the initial stage, and the damage of oxygen free radicals was the major factor in ischemia-reperfusion injury. Taurine had protective effects on every aspect of the damages of hepatic mitochondria in ischemia-reperfusion injury. Conclusion: These studies suggested that taurine as an effective scavenger for oxygen free radicals had very important phgsiological actions.

关 键 词：肝线粒体 保护作用 牛磺酸 缺血再灌注 线粒体 大鼠 

分 类 号：R285[医药卫生—中药学]