CYP1A1基因多态性与肺癌个体易感性研究  

The Relationship between CYP1A1 Genetic Polymorphism and Susceptibility to Lung Cancer

作　　者：周新文[1] 石云[1] 周宜开[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院环境医学研究所,武汉430030

出　　处：《环境与职业医学》2002年第6期355-357,387,共4页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金资助 (编号 :39990 570 )

摘　　要：[目的 ]探讨CYP1A1Msp1和Ile/Val多态性单独或联合作用 ,对肺癌易感性的影响。 [方法 ]以病例一对照研究的方法 ,采用PCR扩增限制酶切法 (PCR -RFLP)和等位基因特异性扩增 (Allele SpecificAmplification ,ASA)检测 92例肺癌病人 (病例组 )和 98例非肿瘤病人 (对照组 )CYP1A1基因Msp1和Ile/Val基因型。 [结果 ]Msp1多态性位点 :具有B和C基因型者患肺癌的危险性是A基因型者的 1 85倍 (χ2 =4 3 6,P <0 0 5 ,OR =1 85 ,95 %CI 1 0 4～ 3 3 0 )。Ile/Val多态性位点 :Val/Val基因型者患肺癌的危险性是Ile/Ile基因型者的 3 3倍 (χ2 =4 12 ,P <0 0 5 ,OR =3 3 ,95 %CI 1 0 2～10 72 )。Ile/Val基因型联合B基因型、C基因型或Val/Val基因型联合C基因型与Ile/Ile基因型联合A基因型相比 ,患肺癌的危险性增加 ,其相对危险度分别为 3 0 9(χ2 =5 81,P <0 0 5 ,95 %CI 1 7～ 9 96) ;4 74(χ2 =4 74,P <0 0 5 ,95 %CI1 11～ 2 0 9) ;5 5 (χ2 =4 42 ,P <0 0 5 ,95 %CI 1 2 7～ 2 3 6)。 [结论 ]CYP1A1基因的B、C和Val/Val基因型可能是肺癌的易感基因型 ,两种易感基因型同时存在 。To explore the effects of combined CYP1A1 Msp1 genotypes with Ile/Val genotypes on susceptibility to lung cancer. Msp1 and Ile/Val genotypes of CYP1A1 gene were detected with the methods of PCR RFLP and allele specific amplification(ASA) in a case control study,including 92 cases of lung cancer and 98 hospital controls. Msp1 polymorphism site:The risk of lung cancer with the individuals of genotype B or genotype C was 1 85 times greater than that with the individual of genotype A ( χ 2=4 36,P<0 05,OR=1 85,95% CI 1 04～3 30 ).Ile/Val polymorphism site:The risk of lung cancer of the individuals with genotype Val/Val was 3 3 times greater than the individual with genotype Ile/Ile ( χ 2=4 12,P<0 05,OR=3 3,95% CI 1 02～10 72 ).The lung cancer risks of individuals with combined Ile/Ile genotype and A genotype were compared with these of individuals combined Ile/Val genotype and B ( χ 2=5 81,P<0 05,95% CI 1 7～9 96 ),the individuals combined Ile/Val genotype and C genotype ( χ 2=4 74,P<0 05,95% CI 1 11～20 9 ) and the individuals combined Val/Val genotype and C genotype ( χ 2=4 42,P<0 05,95% CI 1 27～23 6 ). [Conclusion] The genotype C and genotype Val/Val of CYP1A1 gene may be susceptible to lung cancer,the individuals with two susceptible genotypes were more susceptible to lung cancer.

关 键 词：CYP1A1基因多态性 等位基因特异性扩增 易感性 肺癌 

分 类 号：R734.202[医药卫生—肿瘤]