CHROMATOGRAPHIC SEPERATION AND PURIFICATION OF HUMAN CHORIONIC GONADOTROPIN  

CHROMATOGRAPHIC SEPERATION AND PURIFICATION OF HUMAN CHORIONIC GONADOTROPIN

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作  者:Wang Haifeng Xu Yutai Ren Rougli Tang Lei Wang Hairen 

机构地区:[1]Department of Biology, Shandong University, Jinan 250100

出  处:《Chinese Journal of Reactive Polymers》1995年第1期39-44,共6页中国反应性高分子(英文版)

摘  要:Seperation and purifitation of human chorionic gonadotropion (HCG) in theurine sample of early pregnant women by D3520 resin adsorption chromatographyis repaied. The crude product obtained by DEAE - Cellulose 23 and DEAE Sephadex A50 column chromatography showed a high activity of HCG. Furtherpurifikation of the sample by gel filtration chromatography on a Sephadex G75 column gives a final preparation of 6000~ 6500 IU/mg. The preparation meets therequiTements of the pyrogn test in Chinese Law of Pharmacopoeia.

关 键 词:CHROMATOGRAPHY human chorionic gonadotorpion D3520 resin 

分 类 号:Q579.1[生物学—生物化学] O658.1[理学—分析化学]

 

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