钙、镁离子对淀粉酶催化激活作用的热动力学  

Thermokinetics of Activation of Metal Ions to Amylase Catalyzed Reaction

作　　者：张洪林[1] 于秀芳[1] 张刚[2] 

机构地区：[1]曲阜师范大学化学系,曲阜273165 [2]山东大学微生物技术国家重点实验室,济南250100

出　　处：《物理化学学报》2002年第12期1125-1128,共4页Acta Physico-Chimica Sinica

基　　金：山东省自然科学基金(Y2000B03)资助项目~

摘　　要：利用热活性检测仪测定了无激活剂和有激活剂时淀粉在淀粉酶催化作用下的热功率-时间曲线,借助热动力学理论和对比进度法对曲线进行处理,得到了无激活剂时酶催化反应的米氏常数(Km)和最大速率(vmax),以及有激活剂(Ca2+,Mg2+)时酶催化反应的表观米氏常数(Km1)和最大速率(vmax).通过比较,得到了金属离子对酶催化反应定量的激活效果.Power-time curves ofamylase catalyzed reaction with or without activation were obtained with a 2277thermal activity monitor(Sweden). The data of Km and vmax were calculated for enzyme catalyzed reactions without activation. Data of apparent and vmax were calculated for activation (Ca2+, Mg2+) using thermoki <IMG SRC='IMAGE/01220553.JPG' HEIGHT=11 WIDTH=12>netics theory and reduced extent method. From the relationship between and concentration of metal ions(c), theeffects of the activation were gained for metal ions of amylase catalyzed reaction.

关 键 词：镁离子 催化激活作用 微量热法 热动力学 淀粉酶 催化反应 钙离子 

分 类 号：O643.3[理学—物理化学]