浙江省嘉兴地区多重耐药肺炎克雷伯菌β-内酰胺酶编码基因分子生物学研究  

Encoding genes and genotypes of β-lactamases produced by a multiple resistant Klebsiella pneumoniae

作　　者：俞云松[1] 吕芳芳[1] 宋秀兰 陈亚岗[1] 周伟琳[1] 马亦林[1] 

机构地区：[1]浙江大学医学院附属第一医院,浙江杭州310003 [2]浙江省嘉兴第一人民医院

出　　处：《浙江大学学报（医学版）》2002年第6期457-460,共4页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省医学重点基金资助项目 (2 0 0 0 ZD0 0 2 )

摘　　要：目的 :确定浙江省嘉兴地区临床分离的多重耐药肺炎克雷伯菌 E3株所产β-内酰胺酶编码基因序列及亚型。方法 :肺炎克雷伯菌 E3株经酶抑制剂增强的肉汤稀释法鉴定为产 ESBL s细菌 ,PCR扩增其β-内酰胺酶编码基因片段 ,克隆后双脱氧链终止法测定核苷酸序列并确定亚型 ,重组细菌表型鉴定。结果 :E3株同时产 SHV和TEM型两种β-内酰胺酶 ,其中 SHV基因片段含 812个核苷酸 ,编码 2 6 6个氨基酸 ,为 SHV- 11型β-内酰胺酶 ;TEM基因片段含 973个核苷酸 ,编码 2 88个氨基酸 ,为 TEM- 1型β-内酰胺酶。含 TEM- 1和 SHV- 11编码基因的重组细菌经酶抑制剂增强的肉汤稀释法鉴定为阴性。结论 :E3株肺炎克雷伯菌同时产生 SHV- 11和 TEM- 1两种广谱β-内酰胺酶 ,广谱β-内酰胺酶的种类和数量可影响 ESBLObjective: To determine the sequence of gene for encoding β lactamase produced by Klebsiella pneumoniae E3 isolated from Jiaxing Area in Zhejiang Province. Methods: The Klebsiella pneumoniae strain E3 was identified as an ESBLs producing bacterium by inhibitor potentiated broth dilution test. The gene encoding β lactamase of the strain was amplified by PCR. The purified PCR product was cloned and sequenced by Sanger's dideoxy chain termination composition method. Results: The Klebsiella pneumoniae strain E3 produced both TEM and SHV β lactamases. The SHV encoding gene had 812 nucleotide residues responsible for encoding SHV 11 β lactamase and the TEM encoding gene had 973 nucleotide residues responsible for encoding TEM 1 β lactamase. Conclusion: The Klebsiella pneumoniae strain E3 isolated from a patient in Jiaxing Area in Zhejiang Province is able to produce both TEM 1 and SHV 11 β lactamases.

关 键 词：肺炎克雷伯氏菌 酶学 Β-内酰胺酶类 遗传学 超广谱Β-内酰胺酶 聚合酶链反应 序列分析 

分 类 号：R563.1[医药卫生—呼吸系统]