用微芯片化学反应器实现酶催化化学发光测定葡萄糖的研究  

Studies on β-Glucose Chemiluminescence Detection Catalyzed by GOD with Microchip Chemical Reactor

作　　者：徐溢[1] 

机构地区：[1]重庆大学化学化工学院,光电技术及系统教育部重点实验室,重庆400044

出　　处：《高等学校化学学报》2003年第1期49-51,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 0 0 0 70 0 5 );教育部留学回国人员基金 (批准号 :外教留司 1999-36 3)资助

摘　　要：Glucose oxidization was catalyzed by glucose peroxidase, reactive products peroxides could have Chemiluminescence reaction with luminol. These were applied to detect glucose quantitatively in-situ or on-line by the microreactor with micromixer and FIA system. In this paper, the effects of many factors, such as the characters of microchip, modification of microchip glass surface, pH values of the detecting system, CL intensifying agent and so on, were studied in detail on the glucose detection. A new reliable and portable easily automatable system was set up for the glucose detection.Glucose oxidization was catalyzed by glucose peroxidase, reactive products peroxides could have Chemiluminescence reaction with luminol. These were applied to detect glucose quantitatively in-situ or on-line by the microreactor with micromixer and FIA system. In this paper, the effects of many factors, such as the characters of microchip, modification of microchip glass surface, pH values of the detecting system, CL intensifying agent and so on, were studied in detail on the glucose detection. A new reliable and portable easily automatable system was set up for the glucose detection.

关 键 词：酶催化 葡萄糖 葡萄糖氧化酶 过氧化物酶 微芯片化学反应器 流动注射分析 化学发光 

分 类 号：O629.112[理学—有机化学] Q532.3[理学—化学]