肝癌细胞凋亡相关基因分析  

Differentially expressed cDNA sequences homologous with known genes in apoptotic hepatocellular carcinoma cells

作　　者：曾建新[1] 王文亮[1] 王知力[1] 骆文静[1] 

机构地区：[1]第四军医大学基础部病理学教研室,陕西西安710033

出　　处：《第四军医大学学报》2002年第24期2240-2243,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目 (9470 771 )

摘　　要：目的　克隆肝癌细胞凋亡相关基因 ,分析已知基因同源的cDNA序列 ,探讨三氧化二砷 (As2 O3 )诱导肝癌细胞凋亡的分子机制 .方法　用As2 O3 诱导人肝癌HCC 92 0 4细胞凋亡 ,应用抑制性消减杂效技术克隆凋亡细胞中的差异表达cD NA ,测序并与Genebank中的已知基因序列进行同源性比较 .结果　克隆到 18个与已知基因高度同源的cDNA序列 ,包括与抗氧化损伤、线粒体跨膜运输、细胞骨架系统相关的基因、细胞凋亡相关核蛋白PHLDA1基因、核糖体蛋白L37基因、溶酶体ATP酶基因以及与Ibd1、SMT3、DKFZP4 34J15 4 protein等基因高度同源的序列 .结论　As2 O3 诱导的肝癌细胞凋亡后 ,大量基因表达上调 ,这些基因大多与As2 O3 处理肝癌细胞后的应激反应和细胞凋亡有关 ,提示细胞凋亡是一个有多基因参与调控的复杂过程 .AIM To clone apoptosis associated genes in hepatomacellular carcinoma cells and to explore the molecular mechanisms of apoptosis induced by arsenic trioxide by analyzing the cDNA sequence homologous with the known genes. METHODS Apoptosis was induced by arsenic trioxide (As 2O 3) in HCC 9204 cells and the differentially expressed cDNAs were cloned using suppression subtractive hybridization. Homology searches were performed by BLAST program on internet. RESULTS 18 cDNAs homologous with known genes were cloned. These genes included those involved in anti oxidation stress, transmembrane transport of mitochondria, and cytoskeletal proteins, and those coding for aldolase A, apoptosis associated nuclear protein PHLDA1, ribosomal protein L37, lisosomal ATPase, Ibd1, SMT3, DKFZP434J154 protein, etc. CONCLUSION Expression of many genes increases after the treatment of As 2O 3 in HCC 9204 cells. Most of these genes are stress associated or apoptosis associated. Our findings suggest that apoptosis is a complex process regulated by multi genes.

关 键 词：肝癌 细胞凋亡 相关基因分析 脱噬作用 序列分析 

分 类 号：R735.7[医药卫生—肿瘤]