犬脑内移植大鼠胰岛细胞的凋亡状况  

Rat ICC apoptosis of xenotransplantation into dog brain

作　　者：信照亮[1] 贾延杰[2] 胡海涛[2] 任惠民[2] 

机构地区：[1]西安交通大学第二医院神经外科,陕西西安710004 [2]西安交通大学医学院解剖学教研室,陕西西安710004

出　　处：《第四军医大学学报》2002年第24期2279-2281,共3页Journal of the Fourth Military Medical University

基　　金：陕西省科技攻关项目 (2 0 0 0 G1 4 8)

摘　　要：目的　观察生物半透膜包裹异种胰岛样细胞团(ICCs) ,结合相对免疫特赦部位脑实质内移植的凋亡状况 .为异种胰岛细胞脑内移植可能的临床应用提供实验依据 .方法　SD大鼠ICCs分离、纯化与培养 ,生物半透膜包裹后犬脑实质内移植 .电镜观察移植后的细胞形态 ,胰岛素 TUNEL双标记染色鉴定胰岛β细胞凋亡 ,免疫组化染色观察凋亡相关基因bcl 2和bax的表达 .结果　胰岛素 TUNEL双标记染色证明了胰岛β细胞发生了凋亡 ,电镜下可见典型的凋亡细胞 ,有凋亡小体形成 .移植 1mo和 2mo,白细胞数分别为 2 4± 5和 93± 9(P <0 .0 1) ,白细胞数 /胰岛素阳性细胞数之比为 8± 3和2 0± 8(P <0 .0 1) ,随着移植时间的延长 ,白细胞增多 ,胰岛细胞减少 .Bcl 2和bax基因均有不同程度的表达 ,bax染色更浓 .结论　异种移植后的胰岛细胞死亡与凋亡有关 .半透膜包裹异种ICCs结合免疫特赦部位脑内移植的方法对克服免疫排斥反应有一定的作用 .AIM To study the apoptosis of islets after xenotansplantation in brain enwrapped with the biological semipermeable membrane (BSM) and to provide experimental evidence for clinical xenotransplantation of islet cells in the brain. METHODS Rat islet like cluster cells (ICC) enwrapped by BSM were xenotransplanted into dog's brain. The morphological changes were observed by electronic microscopy. The DNA fragmentations of β cells and the expressions of apoptosis related genes in the implants were identified by insulin TUNEL double staining and immunohistochemistry. RESULTS The β cells in the implants showed a classic presentation of apoptosis by insulin TUNEL double staining and electric microscopy. The expressions of bcl 2 and bax were found, and bax were of more staining density. Leukocytes increased in the implant and the β cells decreased with the time extended ( P <0.01). CONCLUSION The death of islets in xenotransplantation is associated with apoptosis. The method of biological semipermeable membrane combined with the privileged site xenotansplantation helps alleviate the rejection of ICCs implant.

关 键 词：大鼠 胰岛细胞 脑移植瘤 

分 类 号：R739.41[医药卫生—肿瘤]