大鼠精蛋白基因在MEL细胞中的表达  

Expression of Rat Protamine Gene in MEL Cells

作　　者：陈宇[1] 费仁仁[1] 吴小芳[1] 葛晔华[2] 陈晖[1] 马静[2] 韩代书[2] 薛社普[2] 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所,北京100050 [2]Institute of Basic Medical Sciences,CAMS and PUMC,beijing100005

出　　处：《中国医学科学院学报》2002年第6期601-605,共5页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(39670364)

摘　　要：目的研究大鼠精蛋白(RP)基因在MEL细胞中的表达及其对细胞生长的影响。方法构建RP真核表达载体,并转染MEL细胞。分析细胞生长曲线、分裂指数及在半固体培养基中的集落形成率,并分析RNA的转录水平。结果转染细胞中RP得到了表达,RP表达后MEL细胞增殖率减慢,分裂指数降低以及在半固体培养基中的集落减少,RNA转录下降。结论RP的表达对肿瘤细胞有生长抑制作用。Objective To investigate the expression of rat protamine(RP)gene in MEL ce lls and the effect on cell growth.Methods Eukaryotic expression plasmid pCR-3 .1-RP was constructed and transfected into MEL cells.The changes of cell grow th rate,mitotic index and colony-forming rate in semi -solid medium were invest igated.Results Transfected MEL cells showed lower growth rate,mitotic index an d colony-forming rate.Volumes of cells were reduced and reduction of RNA transc ription was observed.Conclusion These results suggest that expression of RP in MEL cell may inhibit the cell growth and proliferation.

关 键 词：精蛋白基因 MEL细胞 红白血病 基因表达 真核表达载体 细胞转染 肿瘤基因治疗 

分 类 号：R733.705[医药卫生—肿瘤]