c-myc反义寡核苷酸对肿瘤细胞表面抗原和对CD_3AK杀伤敏感性的调节作用  

Effects of c-myc antisense oligodeoxyribonucleotides(ASODNS) on the expression of surface antigen of the tumor cells and susceptibility of target cells to CD_3AK attack

作　　者：李兆忠[1] 张玲[1] 王芸[1] 毛海婷[1] 李登华[1] 崔树龄[1] 

机构地区：[1]山东省医学科学院基础医学研究所,济南250062

出　　处：《中国免疫学杂志》2003年第1期29-31,35,共4页Chinese Journal of Immunology

基　　金：山东省自然科学基金资助项目 (Y99C0 6 )

摘　　要：目的 :观察c myc反义寡核苷酸上调人高转移性肺巨细胞腺癌PG细胞表面抗原分子的表达水平 ,提高免疫效应细胞杀伤敏感性的作用和机制。方法 :RT PCR方法检测c mycmRNA表达水平的变化。MTT法检测细胞增殖活性和CD3AK杀伤活性的变化。流式细胞术检测细胞表面抗原表达的变化以及c myc蛋白表达水平的变化。结果 :c myc反义寡核苷酸(1 μmol L) 明显地抑制PG细胞c mycmRNA和蛋白表达水平 ,显著提高细胞表面HLA ABC、ICAM 1分子的表达 ,其表达率分别从 68 44%、38 40 %增高到 83 1 6 %和 42 0 9% (P <0 0 1 )。CD3AK对反义寡核苷酸处理的PG细胞的不同效靶比杀伤活性 ,分别从 40 0 %、65 0 %、74 0 %增高到 52 0 %、74 0 %、91 0 % (P <0 0 1 )。结论 :c myc反义寡核苷酸通过抑制PG细胞c mycmR NA和蛋白表达 ,上调PG细胞表面HLA ABC、ICAM 1分子的表达水平 。Objective:To study the role and mechanism of c myc ASODNS on cell expression of surface antigen of the tumor cells and susceptibility of target cells to immune cells attack.Methods:c myc mRNA was examined by RT PCR.MTT assay was usd to explore the effects of c myc ASODNS on PG cell sentisentity to lysis by CD 3AK effector cells.The expression of HLA ABC,ICAM 1,c myc protein was examined by flow cytometry.Results:When PG cells were treated with ASODNS(1 μmol/L) there was a markably reduction of expression of c myc protein.Expression ratio of HLA ABC and ICAM 1 surface antigen expression ratio of PG cells were enhanced from 68.44%,38.44% to 83.16% and 42.09% respectively( P <0.01).After exposure of PG cells to c myc ASODNS,the susceptitility of PG cells to lysis by CD 3AK was enhanced from 40.0%,65.0%,74.0% to 52.0%,74.0% and 91.0%( P <0.01) at an different effector:target ratio respectively.Conclusion:c myc ASODNS can block and inhibit expression of c myc mRNA and protein,enhance expression of adhesion molecules ICAM 1 and HLA ABC which agument sentisentity of PG cells to lysis by CD 3AK effector cells.

关 键 词：c-myc 反义基因 HLA-ABC ICAM-1 CD3AK 肿瘤 

分 类 号：R730.3[医药卫生—肿瘤]