短短芽胞杆菌及其芽胞对芘的降解  

Biodegradation of Pyrene by Intact Cells and Spores of Brevibacillus brevis

作　　者：刘芷辰[1] 叶锦韶[1] 彭辉[2] 刘则华 邓庭进 尹华 廖丽萍 

机构地区：[1]暨南大学环境学院广东省高校水土环境毒害性污染物防治与生物修复重点实验室,广州510632 [2]暨南大学化学系,广州510632 [3]华南理工大学环境与能源学院工业聚集区污染控制与生态修复教育部重点实验室,广州510006

出　　处：《环境科学》2015年第5期1763-1768,共6页Environmental Science

基　　金：国家自然科学基金委-广东省联合基金重点项目(U0933002);广东省自然科学基金重点项目(S2013020012808);中央高校基本科研业务费专项(2013ZM0126)

摘　　要：考察短短芽胞杆菌(Brevibacillus brevis)菌体及芽胞对四环多环芳烃芘的降解性能.结果表明,菌体5 d内对1 mg·L-1芘的降解率可达53%.菌体和胞内酶降解芘过程中检测到1-羟基芘、9-羟基菲、α-萘酚和β-萘酚这4种单羟基降解产物,在完整菌体降解体系中,产物呈现先积累后下降的趋势,而在胞内粗酶液降解体系中,大分子代谢产物表现为一直积累的趋势,说明B.brevis完整菌体在降解初期并不具备一些中间产物的降解酶,但随着时间推移,可被诱导产生相关酶对生成的新产物进行进一步降解.芽胞悬液在添加芘的无机盐培养基中5 d内萌发生成的营养细胞可达到1.5×109个·L-1,对1 mg·L-1芘的降解率达到15%.Biodegradation characteristics of pyrene by intact cells and spores of Brevibacillus brevis were investigated. The results revealed that the degradation efficiency of 1 mg·L- 1pyrene by B. brevis intact cells reached 53% within 5 d. Four hydroxy metabolites were detected by LC-MS / MS during the degradation of pyrene by intact cells and intracellular enzymes,including 1-hydroxy-pyrene,9-phenanthrol,α-naphthol and β-naphthol. The amount of products first increased and then declined in the degradation system with intact cells,while the macromolecular metabolites showed a continuous accumulation tendency in the degradation system with intracellular enzymes,proving that some degrading enzymes of B. brevis could be induced by the products generated during the degradation,and then the enzymes induced could further degrade these degradation products. The amount of cells germinated from spores in MSM containing pyrene was 1. 5 × 109cells·L- 1,and the degradation rate of pyrene( 1 mg·L- 1) within 5 d reached 15%.

关 键 词：芘 短短芽胞杆菌 胞内酶 芽胞 降解产物 

分 类 号：X172[环境科学与工程—环境科学]