Studies on Membrane Function and Sugar Components of Ultradried Seeds  

超干种子的膜功能与糖组分的研究(英文)

作　　者：汪晓峰[1,2] 景新明[1] 林坚[1] 郑光华[1] 蔡早 

机构地区：[1]中国科学院植物研究所 [2]中国林业大学生物学院,北京100083

出　　处：《Acta Botanica Sinica》2003年第1期23-31,共9页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家重大基础研究项目(G2000046806);国家基金项目(30170100);中国科学院财政部生命科学专项支持~~

摘　　要：The effects of ultradry storage on the activity of ATPase and fluidity of plasma membrane in Chinese cabbage (Brassica pekinensis (Lour.) Rupr.) and elm ( Ulmus pumila L.) seeds were investigated. The results indicated that no significant differences in the activity of ATPase and the micro- viscosity of plasma membrane of ultradried (UD) seeds could be found as compared to the control seeds stored under -20 degreesC, although there was a little adverse effect on the seeds with extreme dehydration. The results were consistent with higher vigor level of UD seeds. This implied that ultradry seed storage could protect the integrity of the membrane, maintain its physiological function and improve the storability of seeds. The relationship between sugar and desiccation tolerance of UD seeds was analysed using the HPLC. The results showed that the ratio of reducing to nonreducing sugar was lower in UD seeds than that in control. The content of sucrose and stachyose in elm and Chinese cabbage UD seeds was related to their desiccation tolerance, while no stachyose was detected in corn (Zea mays L.) seeds. This could be one of the reasons for its sensitivity to desiccation.采用两相法分离种子质膜,研究超干处理对种子质膜ATP酶活力及膜流动性的影响。结果表明,白菜(Brassica pehinensis (Lour )Rupr.)、榆树(Ulmus pumila L.)种子经超干处理后,在人工老化和自然老化条件下,与在-20℃条件下贮藏的种子比较,超干贮藏种子质膜ATP酶活力和微粘度无显著变化,过干贮藏种子的效应虽有所下降,但仍比高含水量室温贮藏的对照种子为好。此结果与超干种子具较高活力水平完全一致,说明超干贮藏种了保持了质膜的生理功能,因此提高了种子的耐藏性。高效液相色谱分析结果显示超干种子中还原糖/非还原糖的比值低于高含水量种子,积累的蔗糖、水苏糖含量与超干种子的耐干力有关。玉米(Zen mays L.)种子中不含水苏糖,这可能是玉米种子较其他种子耐干力下降的原因之一。

关 键 词：ultradry seed storage ATPASE micro-viscosity soluble sugar 

分 类 号：Q945.65[生物学—植物学]