结核分枝杆菌组合DNA疫苗的免疫效果  

Combined Recombinant DNA Vaccine Results in Significant Protection against Mycobacterium tuberculosis

作　　者：潘怡[1] 蔡宏[1] 李淑霞[2] 田霞[1] 李唐[1] 朱玉贤[1] 

机构地区：[1]北京大学生命科学学院,北京100871 [2]新疆畜牧科学院,乌鲁木齐830000

出　　处：《生物化学与生物物理学报》2003年第1期71-76,共6页

基　　金：国家高技术研究发展计划 ( 86 3计划 )资助项目 (No .2 0 0 1AA2 1314 1)~~

摘　　要：以编码结核分枝杆菌分泌蛋白Ag85B、ESAT 6和MPT6 3的基因为插入片段 ,构建核酸疫苗联合免疫小鼠。以原核表达纯化的抗原为检测物 ,检测了抗原特异性的抗体和γ 干扰素的形成。研究表明 ,组合核酸疫苗第 3次免疫后 2 1天 ,实验小鼠血清中Ag85B抗体滴度达到 10 5以上 ,Ag85B抗原刺激产生的特异性γ 干扰素达到 (17.0± 7.0 )u/ml。组合疫苗虽然没有提高小鼠血清中ESAT 6及MPT6 3蛋白的特异性抗体滴度 ,但仍显著刺激产生了两种特异性的 γ 干扰素。攻毒实验表明 ,经组合疫苗免疫后小鼠肺部结核杆菌数量显著下降。肺部切片显示 ,免疫小鼠病理状况较对照组有明显改善。因此 ,研究提示Ag85B等组合核酸疫苗具有较好的结核病预防效果。Three proteins secrected from Mycobacterium tuberculosis, Ag85B, ESAT-6 and MPT63 were seclected as antigens for making combined DNA vaccine by immunizing mice. The immune response induced by the vaccine and its protective efficacy were studied. It was demonstrated that when mice were immunized with the combined DNA vaccine, the titer of antibody for Ag85B in serum increased to more than 105, but the titers of ESAT-6 and MPT63 specific antibodies were undetectable. After the final immunization, the level of IFN-γ specific for Ag85B, ESAT-6 and MPT63 reached (17.0±7.0) u/ml, (6.0±0.8) u/ml and (11.9±8.0) u/ml, respectively. Mice, that were inoculated with the empty eukaryotic expression vector pJW4303 DNA, produced negligible amounts of antigen-specific INF-γ. The combined DNA vaccine resulted also in significantly reduced amount of bacteria in the lungs of experimental mice. Microphotographs showed clearly that these lungs were better protected against Mycobacterium tuberculosis challenge than control mice. The combined DNA vaccine reported in this work shed new lights on the prophylactic protection against tuberculosis.

关 键 词：分泌蛋白 保护力 结核分枝杆菌 组合DNA疫苗 免疫效果 

分 类 号：R392[医药卫生—免疫学]