Influence of Valsartan on myocardial apoptosis in spontaneously hypertensive rats  

缬沙坦对自发性高血压大鼠心肌细胞凋亡的影响

作　　者：李为民[1] 刘巍[1] 孙宁玲[2] 陈源源[2] 虞有智[2] 

机构地区：[1]哈尔滨医科大学第一临床医学院心内科,哈尔滨150001 [2]北京医科大学人民医院心内科,北京100044

出　　处：《Chinese Medical Journal》2002年第3期364-366,共3页中华医学杂志（英文版）

摘　　要：OBJECTIVE: To explore the pathogenic changes of myocardial apoptosis in heart hypertrophy during hypertension and evaluate the anti-apoptosis effect of Valsartan. METHODS: Thirty spontaneously hypertensive rats (SHRs) were divided into two groups: 15 treated with Valsartan (20 mg x kg(-1) x d(-1)) (SHR + Valsartan group), the others with placebo (SHR + placebo group), with 15 normal Wistar rats as control. Systolic blood pressure was measured by the tail-cuff method. The observation period was from 8 to 16 weeks of age. Cardiac apoptosis was evaluated by a Terminal Deoxynucleotidyl Transferase-Mediated dUTP-biotin Nick End Labeling (TUNEL) assay. RESULTS: Mean blood pressure values were 127 +/- 2 mm Hg in controls, 163 +/- 6 mm Hg in the SHR + Valsartan group and 193 +/- 7 mm Hg in the SHR + placebo group at 16 weeks of age, whereas the blood pressure in 8-week-old SHR and Wistar rats were 175 +/- 3 mm Hg and 125 +/- 5 mm Hg, respectively. The ratio of the heart weight over body weight declined in Wistar (3.07 +/- 0.03 mg/g) and SHR + Valsartan groups (3.22 +/- 0.19 mg/g) compared with the SHR + placebo group (4.02 +/- 0.31 mg/g) (P目的　探讨遗传性高血压及心肌肥厚过程中心肌细胞凋亡的意义及AT1受体拮抗剂缬沙坦对其的影响。方法　实验动物为 8周龄自发性高血压大鼠 (SHR) ,分为缬沙坦治疗组 (SHR +缬沙坦组 )和非治疗组 (SHR无药组 ) ,以Wistar鼠作为正常血压对照 ,观察期限为 8- 16周。采用TUNEL染色法检测心肌组织凋亡细胞数。结果　缬沙坦治疗后血压降至 16 3± 6mmHg ,与治疗前 175± 3mmHg及无药组 193± 7mmHg比较显著降低。SHR +缬沙坦组心脏指数 3 2 2± 0 19mg/g ,较SHR无药组 4 0 2± 0 31mg/g显著降低。SHR +缬沙坦组与SHR无药组比较 ,前者TUNEL阳性细胞数显著减少至接近Wistar组。结论　心肌细胞凋亡是代偿性心肌肥厚发展为心力衰竭的可能机制之一。高血压病早期缬沙坦在降压同时有效抑制心肌细胞凋亡。

关 键 词：Animals Antihypertensive Agents Apoptosis CARDIOMEGALY Hypertension Myocardium RATS Rats  Inbred SHR Rats  Wistar TETRAZOLES VALINE 

分 类 号：R544.1[医药卫生—心血管疾病] R965[医药卫生—内科学]