Effect of NF-κB on the induction of PDGF-B transcription by angiotensin Ⅱ in the ECV304 cell line  

作　　者：李皓[1] 尹鸿操[1] 张华[1] 曹心宜[1] 王宗立[1] 佘铭鹏[1] 

机构地区：[1]中国医学科学院

出　　处：《Chinese Medical Journal》2002年第3期433-438,共6页中华医学杂志（英文版）

基　　金：supported by the National Natural Science Foundation of China(No．39730220)．

摘　　要：Objective To examine the effect of angiotensin Ⅱ (Ang Ⅱ) on nuclear factor-kappa B (N F-κB) activation in human endothelial cell line ECV304 and the molecular mechanism by which Ang Ⅱ activat es NF-κB. Methods ECV304 cells were transiently cotransfected with an NF-κB/ luciferase reporter gene and inactive NF-κB-inducing kinase (NIK), IκB kinase α (IKK_α), I κB kinase β (IKK_β) mutants or vectors, respectively. The effect on NF- κB was detected by using an electrophoretic mobility shift assay (EMSA) and overex pression of the mutants enabled blocking of reporter gene activation induced by Ang Ⅱ. With immunofluorescence and immuno-electronic microscope techniques, including confocal microscopy and gold particle labeled electronic microscopy, d efinite cytoplasmic-to-nuclear translocations of NF-κB activation were detec ted using subunits p50 and p65 induced by Ang Ⅱ. Results The translocation of p50 in nuclei was highly remarkable 2 hours after Ang Ⅱ st imulation, and the activity was somewhat reduced 6 hours after stimulation to th e 18th hour. Northern blot also showed PDGF-B mRNA increased by stimulation of Ang Ⅱ for 18 hours. Conclusion Ang Ⅱ is effective in stimulating NF-κB activation through a pathway dependen t on NIK, IKK_α and IKK_β, and induces PDGF-B transcription in the endothel ial cell line, ECV304.

关 键 词：angiotensin Ⅱ · nuclear factor-kappa B · endothelial cell · PDGF-B 

分 类 号：R363[医药卫生—病理学]