Prolongation of functional life-span of neutrophils by recombinant verotoxin 2  

重组V2毒素延长中性粒细胞的功能性生命期限(英文)

作　　者：刘佳佳[1] 何延正[2] 何涛[1] 张忠杰[2] 赤星透 近藤启文 钟森[2] 

机构地区：[1]泸州医学院中心实验室,泸州646000 [2]泸州医学院附属医院外科,泸州646000 [3]日本北里大学医学部内科

出　　处：《Chinese Medical Journal》2002年第6期900-903,共4页中华医学杂志（英文版）

摘　　要：OBJECTIVE: Verotoxin-producing Escherichia coli (VTEC) strains of serotype O157 : H7 have been implicated in a wide spectrum of diseases, including blood diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS). To further explore the pathological role of verotoxin (VT) in HUS and other VTEC associated diseases, we investigated the effects of recombinant verotoxin 2 (rVT2) on the biological activity of neutrophils. METHODS: The technique of flow cytometry, a fluorescent probe 2,7-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF/AM), and the assay of reduced cytochrome c to detect superoxide production were used in this study. RESULTS: gammaVT2 significantly inhibited spontaneous apoptosis in neutrophils. Neutrophils with prolonged survival due to gammaVT2 maintained various biological functions, such as the expression of adhesion molecules (shading CD62L and raising CD11b/CD18), adherence to human umbilical vein endothelial cells (HUVECs), and generation of superoxide (O(2)(-)). CONCLUSION: Prolongation of the functional life-span of neutrophils by gammaVT2 may accelerate inflammatory responses at sites of inflammation. This may play a crucial role in neutrophil-mediated tissue injury in HUS and other VTEC-associated diseases.Objective Verotoxin-producing Escherichia coli (VTEC) strains of serotype O157∶H7 have been implicated in a wide spectrum of diseases, including blood diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS). To further explore the pathological role of verotoxin (VT) in HUS and other VTEC-associated diseases, we investigated the effects of recombinant verotoxin 2 (rVT2) on the biological activity of neutrophils.Methods The technique of flow cytometry, a fluorescent probe 2,7-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF/AM), and the assay of reduced cytochrome c to detect superoxide production were used in this study. Results rVT2 significantly inhibited spontaneous apoptosis in neutrophils. Neutrophils with prolonged survival due to rVT2 maintained various biological functions, such as the expression of adhesion molecules (shading CD62L and raising CD11b/CD18), adherence to human umbilical vein endothelial cells (HUVECs), and generation of superoxide (O 2 -). Conclusion Prolongation of the functional life-span of neutrophils by rVT2 may accelerate inflammatory responses at sites of inflammation. This may play a crucial role in neutrophil-mediated tissue injury in HUS and other VTEC-associated diseases.

关 键 词：Apoptosis Cell Adhesion Endothelium  Vascular Humans NEUTROPHILS Recombinant Proteins Shiga-Like Toxin II SUPEROXIDES 

分 类 号：R392[医药卫生—免疫学]