Prognostic value of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in bladder carcinoma  

MMP-2和TIMP-2在膀胱癌组织中的表达及对预后的判断(英文)

作　　者：许克新[1] 侯树坤[1] 杜志军[1] 

机构地区：[1]北京大学人民医院泌尿外科,北京100044

出　　处：《Chinese Medical Journal》2002年第5期743-745,155,共3页中华医学杂志（英文版）

摘　　要：OBJECTIVES: To detect the level of matrix metalloproteinase-2 (MMP-2) mRNA and the tissue inhibitor of metalloproteinase-2 (TIMP-2) mRNA in bladder transitional cell carcinoma (BTCC), and to estimate the prognosis for bladder tumor based on the quality and quantity of MMP-2 and TIMP-2 mRNA. METHODS: Thirty-five samples of human BTCC and 15 normal fresh bladder tissues were studied by RT-PCR analysis followed by computer-assisted image analysis. RESULTS: The level of the MMP-2 mRNA in BTCC was significantly increased compared with that in normal bladder epithelium. The positive rates of MMP-2 and TIMP-2 mRNA were 71.4% and 65.7% in BTCC, and 66.7% and 60.0% in the normal bladder wall. The expression intensity of the MMP-2 mRNA by image analysis tended to increase with tumor grading and staging, which showed statistical significance. Similarly, the MMP-2 to TIMP-2 ratio also showed statistically significant difference between normal bladder tissue and bladder carcinoma (P目的　检测人膀胱癌组织中MMP 2及TIMP 2的基因表达 ,并结合膀胱癌的分期、分级判断癌恶性度及表达对预后的影响。方法和结果　采用RT PCR的方法 ,检测人BTCC中MMP 2、TIMP 2的基因表达 ,应用计算机图象分析判断MMP 2及TIMP 2的基因表达率分别为 71 4 %和 6 5 7% ;而正常膀胱上皮组织中检测出的MMP 2、TIMP 2的基因表达呈增加趋势 ;而MMP 2 /TIMP 2的比率在正常膀胱组织和膀胱癌之间亦有显著性差异。结论　MMP 2的基因表达以及MMP 2 /TIMP 2的比率对判断BTCC的浸润及预后有一定意义。

关 键 词：ADULT Aged Aged  80 and over BLADDER Bladder Neoplasms FEMALE Gelatinase A Gene Expression Regulation  Neoplastic Humans Male Middle Aged Neoplasm Staging Predictive Value of Tests Prognosis RNA  Messenger Research Support  Non-U.S. Gov't Tissue Inhibitor of Metalloproteinase-2 

分 类 号：R737.14[医药卫生—肿瘤]