Experimental study on mechanism and rarity of metastases in skeletal muscle  

骨骼肌转移瘤罕见性及其机理的实验研究(英文)

作　　者：罗成华[1] 蒋彦永[1] 李向红[2] 刘永学[3] 

机构地区：[1]解放军总医院普通外科,北京100853 [2]解放军总医院病理科,北京100853 [3]北京放射医学研究所,北京100850

出　　处：《Chinese Medical Journal》2002年第11期1645-1649,148-149,共5页中华医学杂志（英文版）

摘　　要：OBJECTIVE: To investigate the reasons for the rarity of metastases in skeletal muscle. METHODS: By injecting tumor cells (Walker256 rat carcinosarcoma) through the iliac artery (experimental group) and the tail vein (control group), animal models of blood-borne metastases were established. The quadriceps femoris muscle and lungs were observed grossly and microscopically. Immunohistochemistry was applied to investigate the expression of vascular cell adhesion molecule-1 (VCAM-1) in the microvascular endothelium of these organs. Primary culture of rat skeletal muscle cells was established and conditioned medium (MCM) was collected. Effects of MCM on several tumor cell lines and the biochemical characteristics of skeletal muscle delivered tumor factor(s) were tested by MTT assay. Apoptosis and morphological examination were carried out to investigate the antitumor mechanisms of MCM. RESULTS: In the experimental group, there were no definite metastases observed in muscle cells. In the control group, lung metastases were present in the lungs of all rats that were sacrificed at the 14th day or died spontaneously (17 rats in all). There was no significant difference between the increase in VCAM-1 in quadriceps femoris muscle 7 days after iliac artery injection and that in lungs 7 days after tail vein injection (P > 0.05). In vitro studies showed that the proliferation of tumor cell lines of mouse SP2/0 myeloma, rat Walker256 carcinosarcoma or human chronic granulocytic leukemia K562, human acute lymphatic leukemia HL-60, LS-174-T colon adenocarcinoma, PC3-M prostatic carcinoma and lung giant cell carcinoma with different metastatic potency (PLA801-C with low metastatic potency, PLA801-D with high metastatic potency) was significantly inhibited when cultured with MCM (P目的　探讨骨骼肌转移瘤罕见性的机理。方法　经Wistar大鼠髂动脉、尾静脉注入Walker2 5 6癌肉瘤细胞 ,建立恶性肿瘤经血循环向骨骼肌及肺转移的动物模型 ,并进行了大体及组织学观察。用免疫组化方法观察了血管内皮细胞粘附分子 (VCAM 1)在这些器官中的表达。原代培养的新生Wistar大鼠骨骼肌细胞 ,用MTT法分析骨骼肌细胞条件培养液 (skeletalmuscleconditionedmedium ,MCM)对不同肿瘤细胞系的体外抑瘤作用 ,并观察了骨骼肌源性抑瘤物的生物化学特性 ,及其对肿瘤细胞凋亡及形态学方面的影响。结果　实验组经髂动脉注入瘤细胞后 ,肌纤维间无实验性转移灶形成 ;对照组经尾静脉注入瘤细胞后 ,14天处死及自然死亡鼠全部形成多发的实验性肺转移灶 (共 17只 )。实验组大腿骨骼肌微血管内皮VCAM 1的表达在注瘤第 7天以后显著上升 (P <0 0 0 1) ,对照组肺微血管内皮VCAM 1在注瘤第 7天以后亦显著上升 (P =0 0 18) ,且实验组大腿骨骼肌与对照组肺微血管内皮细胞VCAM 1阳性率无显著差异 (P >0 0 5 )。体外研究表明 ,MCM与哺乳动物源性肿瘤细胞 (小鼠骨髓瘤SP2 /0 ,Wistar大鼠癌肉瘤Walker2 5 6 ) ,人源性白血病细胞 (人慢性粒细胞白血病K5 6 2 ,人急性淋巴细胞白血病HL6 0 )、实体瘤细胞 (人结肠腺癌细胞LS

关 键 词：Animals Cell Division Humans Immunohistochemistry Muscle Neoplasms Muscle  Skeletal RATS Rats  Wistar Tumor Cells  Cultured Vascular Cell Adhesion Molecule-1 

分 类 号：R738.7[医药卫生—肿瘤]