Aloin,cinnamic acid and sophorcarpidine are potent inhibitors of tyrosinase  

芦荟素、肉桂酸、苦参碱对酪氨酸酶的抑制作用(英文)

作　　者：谭城[1] 朱文元[1] 鲁严[1] 

机构地区：[1]南京医科大学第一附属医院皮肤科,南京210029

出　　处：《Chinese Medical Journal》2002年第12期1859-1862,153,共4页中华医学杂志（英文版）

基　　金：ThisresearchwassupportedbyNaturalScienceFoundationofChina(No .39870 70 1)

摘　　要：OBJECTIVE: To evaluate the effects of aloin, cinnamic acid and 15 other kinds of natural chemicals on the activity of tyrosinase, in order to provide lightening agents in the treatment of hyperpigmentation disorders and cosmetic additives. METHODS: Tyrosinase activity was estimated by measuring the oxidation rate of L-dopa. Inhibition of the enzyme was deduced according to the Lineweaver-Burk plots compared to the control. RESULTS: Cadabine, paeonal, farrerol, evodin, cinnamic acid, aloin and sophorcarpidine had different levels of inhibition of tyrosinase. The inhibitory rates of cinnamic acid (2 mmol/L, 0.5 mmol/L), aloin (2 mmol/L) and the rest were significantly higher than that of hydroquinone (0.5 mmol/L) (P目的　研究芦荟素、肉桂酸、苦参碱等 15种单体化学物对酪氨酸酶活性的影响 ,判定其抑制类型 ,从而为色素增加性皮肤病和外用皮肤脱色剂提供药物。方法　选用酪氨酸酶多巴速率氧化法体外测定药物干预前后酪氨酸酶活性 ,计算酪氨酸酶抑制率 ,分别作出Lineweaver Burk曲线 ,推断其抑制类型。结果　 15种中药单体化学物中 ,芦荟素、肉桂酸和苦参碱能显著抑制酪氨酸酶活性 ,其中芦荟素 (2mmol/L)、苦参碱 (2mmol/L和 0 5mmol/L)酪氨酸酶的抑制率高于氢醌 (0 5mmol/L) (P <0 0 5 )。结论　芦荟素、肉桂酸、苦参碱均可下调酪氨酸酶活性。苦参碱属于酪氨酸酶非竞争性抑制 ,芦荟素、肉桂酸属于混合型抑制剂。

关 键 词：CINNAMATES Cosmetics EMODIN Enzyme Inhibitors Humans HYPERPIGMENTATION Monophenol Monooxygenase Plant Preparations Research Support  Non-U.S. Gov't 

分 类 号：R285[医药卫生—中药学]