Establishment of a C57BL/6N mouse model of giardiasis  

蓝氏贾第鞭毛虫C57BL／6N小鼠动物模型的建立

作　　者：卢思奇[1] 王凤云[1] 罗晓冰[2] 陈小宁[2] 

机构地区：[1]首都医科大学细胞生物系寄生虫教研室,北京,100054 [2]承德医学院寄生虫教研室,河北承德,067000

出　　处：《Chinese Medical Journal》2002年第10期1453-1456,共4页中华医学杂志（英文版）

基　　金：北京市自然科学基金，河北省自然科学基金

摘　　要：OBJECTIVE: To establish a C57BL/6N mouse model infected with Giardia lamblia (G. lamblia) isolates from human origin. METHOD: Two groups of C57BL/6N mouse were inoculated with purified cysts of two G. lamblia isolates (CD and XZ) by gavage separately. Patterns and curves of cyst excretion of the infected mice were observed and summarized. Histopathological changes of the small intestines of the infected mice were observed. RESULTS: Thirty-six mice receiving 1 x 10(4) cysts each were all infected. The C57BL/6N mouse showed high susceptibility to G. lamblia infection. There was no notable distinction between the two groups of the mice infected by the cysts of CD and XZ isolates. Cyst excretion occurred with intermittence. Of 36 infected mice, 32 (89%) passed cysts intermittently and 4 (11%) others persistently. The latent period of cyst excretion was 0 - 3 days p.i. (post-inoculation). The interruption of cyst excretion ranged from 12 to 20 days p.i. The fastigium of the cyst excretion was on day 6 p.i. The peak count of the cysts passed during a 2 h collection period was 2.3 x 10(7)/g fecal specimen. Edema, inflammation, cell infiltration, small blood vessels congestion, mitotic figures and mucosa necrosis appeared in sections of intestines. CONCLUSION: C57Bl/6N mouse is a suitable animal model of G. lamblia.目的建立人源蓝氏贾第鞭毛虫C57BL/6N小鼠动物感染模型.方法用分离自河北和江苏两地蓝氏贾第鞭毛虫感染者的包囊(河北株,CD和江苏株,XZ)分别感染两组C57BL/6N小鼠,收集受染鼠粪便,用蔗糖梯度离心法分离纯化包囊,计算排囊数量 ,分析排囊规律;观察小肠病理组织学改变.结果 C57BL/6N小鼠对此2 株蓝氏贾第鞭毛虫具有很高的易感性,接受具有活力的1×104个包囊/只的36只小鼠,均获得感染,其中呈间歇性排囊者为32只(88.9%),连续性排囊者4只 (11.1%).排囊潜伏期为接种后第3d,高峰期为第7d.2小时所排粪便含包囊数最高可达2. 3×107个/g粪. 小肠粘膜出现表面分泌物增加,间质充血、水肿,炎性细胞浸润,粘膜坏死脱落等病理变化.结论 C57BL/6N小鼠可用做建立蓝氏贾第鞭毛虫感染的良好动物模型.

关 键 词：Disease Models  Animal Animals GIARDIASIS Humans MICE Mice  Inbred C57BL Research Support  Non-U.S. Gov't 

分 类 号：R-332[医药卫生]