The effect of FasL expression on pancreatic islet allografts  

配体表达对同种胰岛移植的影响(英文)

作　　者：詹文华[1] 蔡世荣[1] 汪建平[1] 何裕隆[1] 郑章清[1] 彭俊生[1] 

机构地区：[1]中山医科大学附属第一医院外科,广州510080

出　　处：《Chinese Medical Journal》2002年第7期1006-1009,146,共4页中华医学杂志（英文版）

基　　金：ThisstudywassupportedbytheNationalNaturalScienceFundationofChina (No 3 9770 72 6)

摘　　要：OBJECTIVE: To investigate the immune privilege induced by the Fas ligand (FasL) expressed by cotransplanted testicular Sertoli cells in islet allografts, and the effect of FasL gene transfection on islet cells in pancreatic islet allografts. METHODS: Allogeneic islets and testicular cells were cotransplanted into diabetic recipients.Pancreatic islets were infected with the recombinant adenovirus, AdV-FasL, and transplanted into diabetic recipients. Allograft survival, islet function, apoptosis of infiltrative lymphocytes in allografts and gene transfected islet allografts were analyzed. RESULTS: All animals receiving islet allograft alone returned to a diabetic state in a few days (mean survival time 6.3 +/- 0.6 days). When the quantity of testicular cells cotransplanted with islets increased to 1 x 10(7), all animals remained normoglycemic throughout the follow-up period (60 days). FasL expression by cotransplanted Sertoli cells induced apoptosis of activated lymphocytes. Rejection of allografts in the FasL gene transfer group was accelerated and allograft survival was shortened to 3.4 +/- 0.2 days (P目的　探究睾丸细胞FasL表达能否对共移植的胰岛移植物提供免疫豁免作用以及胰岛细胞FasL基因转染对同种胰岛移植的影响。方法　将同种大鼠胰岛及睾丸细胞同时移植于糖尿病受体 ,重组腺病毒AdV FasL感染胰岛细胞后移植 ,观察移植物存活情况、胰岛功能 ,并检测移植物内浸润淋巴细胞以及基因转染胰岛细胞凋亡情况。结果　单纯移植胰岛组平均存活期为 (6 3± 0 6 )d。与胰岛细胞同时移植的睾丸细胞数增加至 1× 10 7时 ,存活期大于 6 0d(P <0 0 5 )。表达FasL的睾丸细胞在移植物内诱导浸润淋巴细胞凋亡。FasL基因转染组出现排斥加速 ,存活期缩短至 (3 4± 0 2 )d。FasL转染的胰岛细胞在移植后 2 4h见FasL表达 ,4 8h表达增强 ,移植后FasL转染胰岛细胞凋亡。结论　表达FasL的睾丸细胞与胰岛同时移植可诱导活化的淋巴细胞凋亡 ,使胰岛移植物获得免疫豁免、存活期延长 ,但通过FasL基因转染使胰岛细胞直接表达FasL引起胰岛细胞凋亡和粒细胞浸润 ,导致排斥加速。

关 键 词：Islets of Langerhans Transplantation Animals Apoptosis Immunohistochemistry Male Membrane Glycoproteins RATS Rats  Wistar Research Support  Non-U.S. Gov't Reverse Transcriptase Polymerase Chain Reaction TRANSFECTION Transplantation  Homologous 

分 类 号：R657.5[医药卫生—外科学]