Tissue tropism of the TTV in experimentally infected rhesus monkeys  

TT病毒在恒河猴实验感染的组织嗜性(英文)

作　　者：肖红[1] 骆抗先[1] 杨守昌[1] 王燕军[1] 梁蔚芳[1] 

机构地区：[1]第一军医大学南方医院感染内科,广州510515

出　　处：《Chinese Medical Journal》2002年第7期1088-1090,156,共3页中华医学杂志（英文版）

摘　　要：OBJECTIVE: To determine whether the transfusion-transmitted virus (TTV) is hepatotropic. METHODS: Total DNA was extracted from various tissues of 5 experimentally infected Rhesus monkeys during the viremic period. A dot hybridization was done with viral double stranded DNA probes or single antisense probes. RESULTS: The double-stranded probe was hybridized with DNA from the liver, bone marrow, spleen,stomach, small intestine and colon. The single-stranded antisense probe was hybridized with DNA from the liver, small intestine and bone marrow of all 5 monkeys, but not with that from other tissues. CONCLUSIONS: As the viral genome was of negative polarity, the plus-stranded fragment identified in our study might be a replicative intermediate, and was only demonstrated in the liver, small intestine, and bone marrow by dot blot hybridization with single-stranded antisense probes. It is suggested that the TTV replicates in the liver, bone marrow and small intestine, and TTV might be hepatotropic.目的　了解TT病毒的嗜肝性。方法　 5只实验感染病毒的恒河猴血症期间 ,采集各种组织 ,抽提各组织DNA ,分别用病毒双链探针或单链负义探针 ,与吸附在纤维膜上的组织DNA进行斑点杂交。结果　双链探针与肝、骨髓、脾、胃、小肠和大肠杂交阳性 ,表明各该组织中都有无包膜DNA病毒的存在。单链负义探针与各该组织DNA杂交 ,仅肝、骨髓和小肠为阳性 ,表示存在可能作为病毒复制中间体的正义单链DNA。结论　肝、骨髓和小肠可能是病毒的复制场所 ,故TT病毒可能具嗜肝性。

关 键 词：ANIMALS Bone Marrow DNA Virus Infections DNA  Viral Intestine  Small Liver Macaca mulatta Torque teno virus Virus Replication 

分 类 号：R363[医药卫生—病理学]