兔眼晶状体再生模型的建立及观察  

To Establish and Observe the Experimental Lens Regeneration Model in Rabbits

作　　者：柳夏林[1] 张新愉[1] 刘奕志[1] 刘欣华[1] 

机构地区：[1]中山大学中山眼科中心,广州510060

出　　处：《眼科学报》2002年第4期230-234,248,共6页Eye Science

基　　金：国家自然科学基金(30070802);国家教委博士点基金(9949)

摘　　要：目的:建立兔眼晶状体再生模型,探讨晶状体上皮细胞生长及晶状体再生的机制。方法:在8只新西兰白兔兔眼上行超声乳化晶状体吸除术,建立晶状体囊袋模型,术后裂隙灯观察晶状体再生并行再生晶状体的组织学检查。结果:术中撕囊口较小并有相对完整囊袋结构的实验兔眼可观察到晶状体再生;再生晶状体周边及前囊下有较好的透明性,越接近中央及后囊越混浊;组织学研究表明再生晶状体的赤道部晶状体上皮细胞增殖分化形成晶状体纤维,中央区LEC未参与增殖分化;再生晶状体的混浊部位其晶状体纤维排列混乱;囊膜皱折区有大量细胞增殖。结论:保留晶状体囊袋空间结构的相对完整性,可使兔眼晶状体再生;晶状体再生是赤道部晶状体上皮细胞增殖分化形成,囊膜形态结构影响细胞的增殖和分化。眼科学报 2002;18:230-234,248.Purpose: To establish the lens regeneration model in New Zealand rabbits, observe the lens regeneration process to investigate the mechanism of lens epithelial cell growth and lens regeneration. Methods: Lens extraction by endocapsular phacoemulsification was performed on 8 experimental eyes in New Zealand albino rabbits weighing approximately 1. 5 - 2. 5kg. To restore a relative intact capsule bag, a little needle tip was inserted through the limbal wound to perform a 2mm-lined anterior capsulotomy, which just permitted the insertion of a 21G phacoemulsification tip. Phacoemulsification extraction was applied to remove all lens cortical material and then Healon was injected intracapsularly to distend the capsule bag. With siitlamp, the procedure of lens regeneration were observed and taken photographs. A histologic study on regenerated lens were also performed. Results: There were 3 different results on experimental eyes according to the size of anterior capsulotomy in surgical procedure. In 4 eyes, when the size of anterior capsulotomy is approximately 2mm and the capsule bag is relatively intact, lens regeneration occured and it has a relatively normal translucent cortex and central nuclear opacity. Among other 4 surgical eyes, the lower half part of lens regeneration was still observed in one eye though the upper half of anterior capsule was damaged, whereas in other 3, owing to the whole anterior capsule lack nearly, lens regrowth was not noted in areas of pupil under the siitlamp biomicroscopy observation and the posterior capsule appeared as gray-white membrane opacity. Histologic study showed that lens epithelial cells in equatorial zone proliferate and differentiate into lens fibers and continues to refill the whole capsule bag. Lens fiber alignment was regular in the periphery of regenerated lens, whereas in the center opacity nuclear, the bulk of the regenerated lens material consisted of an eosinophilic disorganized lens fibers. In addition, there were much of irregular cellular proliferatio

关 键 词：晶状体 再生 动物模型 超声乳化晶状体吸除术 

分 类 号：R-332[医药卫生]