视网膜消化铺片的免疫荧光染色探讨  

The Study of Immunofluorescence on the Retinal Digest Preparations

作　　者：林健贤[1] 白宁艳[1] 唐仕波[1] 张平[1] 冯官光[1] 张文忻[1] 

机构地区：[1]中山大学中山眼科中心,广州510060

出　　处：《眼科学报》2002年第4期246-248,共3页Eye Science

基　　金："广东省眼科视觉科学重点实验室"专项基金资助(B60105)

摘　　要：目的:探讨视网膜消化铺片联合免疫荧光染色技术的可行性。方法:正常Wistar大鼠和链尿佐菌素(Streptozotocin,STZ)诱导的糖尿病2周大鼠各5只。经心脏灌注后,取右眼视网膜作胰酶消化铺片,并在铺片上进行血管细胞粘附分子-1(Vascular celladhesion modecule-1,VCAM-1)单克隆抗体免疫荧光染色。结果:大鼠视网膜消化铺片可以清晰看到视网膜血管走行、分布,正常大鼠未见VCAM-1阳性表达,糖尿病2周组视网膜血管壁见VCAM-1阳性细胞表达。结论:视网膜血管铺片联合免疫荧光染色技术是可行的。眼科学报 2002;18:246-248.Purpose: To study the technique of immunofluorescence staining on the digested retina preparations. Methods: Retinas of 5 normal Wistar rats and 5 STZ diabetic Wistar rats of were used to make digested retina preparations and carry out immunofluorescence staining with the vascular cell adhesion molecule-1 (VCAM-1). Result: The retinal vessels were clearly observed; There were a lot of positive cells in the retinal preparations of the diabetic Wistar rats, while there were no positive cells in the retinal preparations of the nomal rats. Conclusion: It is feasible to carry out immunofluorescence staining on the digested retina preparations. Eye Science 2002; 18: 246 - 248.

关 键 词：视网膜 铺片 免疫荧光 血管细胞粘附分子 糖尿病性视网膜病变 

分 类 号：R770.43[医药卫生—眼科]