几株Bt菌株对紫外线的抗性探讨  

Resistances of Several Strains of Bacillus Thuringiensis to UV Light

作　　者：王卫国[1] 赵永亮[1] 葛中巧[1] 包东武[2] 杨增茹[2] 

机构地区：[1]南阳理工学院生物与化学工程系 [2]南阳理工学院医学院,河南南阳473002

出　　处：《南阳师范学院学报》2002年第6期67-69,共3页Journal of Nanyang Normal University

基　　金：河南省教育厅自然科学基金资助项目 (No .97112 68)

摘　　要：采用不同照射时间的紫外光 (UV)对 1株野生型Bt菌株及 3株Bt菌株Bt0 0 1、Bt2 0 0、Bt0 87进行照射处理后再置于双碟上培养 16h ,结果表明 ,Bt野生菌株在UV处理 3min后已基本全部失活 ,而Bt0 0 1、Bt2 0 0、Bt0 87在UV处理 8min后仍有活性。从UV处理时间的长短及平板上出现菌落数的多少得知 ,尽管菌株Bt0 0 1、Bt2 0 0、Bt0 87对UV的抗性都比野生Bt菌株强得多 ,但并不相等。其中 ,Bt2 0 0相对弱些 ,UV处理 9min后已无菌落 ;Bt0 87最弱 ,UV处理 8min后已无菌落 ;而菌株Bt0 0 1对UV的抗性最强 ,UV处理 13min后仍有菌落出现。这说明不同的Bt菌株 ,其遗传背景是不同的。另外 ,Bt0 0 1经紫外线照射 9min ,培养 16h后发现有 9个菌落发生明显的变异 ,菌落呈棕黄色 ,菌落明显比原始菌落小。涂片 ,油镜下观察其菌体要比原始菌体小 。One wild Bacillus thuringiensis strain which was i solated from the soil with the method of Sodium acetate-Antibiotics and three strains such as Bt00l? Bt200?Bt087 were cultured for l6 hours on solid media after dealing with UV lig ht. The results indicated that the wild-type Bt was dead after lighting by UV on ly for 3 minutes,and Bt001?Bt200?Bt087 were active after lighting by UV for 8 minutes. Although their resistance to UV of Bt001?Bt200?Bt087 is greatly supe rior to the wild-type Bt,they are not equal. Bt200 is relatively weak,its colon ies disappeared after lighting by UV for 9minutes,Bt087 is weakest, its colonie s disappeared after lighting by UV for 8 minutes,Bt001 is strongest among them ,its colonies can still appear after lighting by UV for l3 minutes.In addition ,one mutant was discovered from the strain Bt001 lit by UV for 9 minutes.

关 键 词：BT菌株 苏云金芽孢杆菌 抗紫外线性 杀虫剂 菌体 苏云金杆菌毒素蛋白 降解 

分 类 号：S482.39[农业科学—农药学]