富硒米提取液对EB病毒转化脐血B淋巴细胞及EBV早期抗原表达的影响  

Influence of Selenium-Rich Rice on T ransformation of Umbilical Blood B Lymphocytes by Epstein-Barr Virus and Epstein-Barr Virus Early Antigen Expression

作　　者：简少文[1] 梅承恩[1] 梁永能[1] 李端[1] 陈巧伦[1] 罗慧玲[1] 李永强[1] 蔡体育[1] 

机构地区：[1]中山大学肿瘤防治中心肿瘤研究所,广东广州510060

出　　处：《癌症》2003年第1期26-29,共4页Chinese Journal of Cancer

摘　　要：背景与目的:硒是人体必需的微量元素,具有抗氧化、抗衰老和抑制肿瘤细胞增殖的作用,为进一步探讨硒在肿瘤防治中的作用,我们培育出富含有机硒的大米,现研究富硒米对EB病毒激发脐血B淋巴细胞转化及对Raji细胞EB病毒早期抗原表达的影响。方法:①用富硒米及普通米提取液以1∶4、1∶8的浓度分别加到制备好的EB病毒液中,加入脐血单个核细胞进行淋巴母细胞转化培养实验,计算细胞转化抑制率;②Raji细胞经正丁酸及巴豆油联合诱导的同时加富硒米提取液共同培养,以间接荧光法,经伊文氏蓝复染观察计算EBV-EA抗原阳性率和抑制率。结果:含硒0.11μg/ml(1∶8稀释)的富硒米提取液显著抑制EB病毒对脐血B淋巴细胞的转化,抑制率为83.4%,明显高于普通米提取液的抑制率61.3%(P<0.05)。富硒米提取液对Raji细胞EB病毒早期抗原(earlyantigen,EA)表达有明显抑制作用,0.016、0.078、0.388μg/ml硒浓度组对EA抗原抑制率分别为2.85%、12.88%、20.75%。结论:富硒米提取液对EB病毒转化脐血B淋巴细胞和对Raji细胞EB病毒早期抗原均有明显的抑制作用。BACKGROUND &OBJECTIVE:Selenium(Se ),an antioxidant,is an essential trace element to human b ody.It can be used as an anti-ag ing ag ent and a tumor cell proliferation inhib itor.To further investig ate the eff ect of selenium in cancer prevention,the authors ob served the influence of Se-rich rice extract on the transformation of umbilical b lood B lymphocytes stimulated by Eps tein-Barr virus (EBV)and expression of EBV early antig en(EBV-EA)in Raji cells.METHODS :(1)Se-rich rice and g eneral rice extrac t(dilution of 1∶4or 1∶8)were added to mixture of EBV,and then umbi lical blood mononuclear cells were added.Lymphoblasts transformatio n test was then performed.The inhibi tion rate of B lymphocytes transformation was calculated.(2)Raji cells stimulated by butyrate and croton oil were incubat ed with Se-rich rice extract.The EBV-EA positive expression rate and the inh ibition rate were counted using indi rect immunolog ical fluescence method.RESULTS :The transformation of umbilical blood B lymphocytes stimulated by EB V was sig nificantly inhibited by Se-rich rice extract at a concentration of 0.11g /ml (1∶8diluted).The inhibition rate was 83.4%(P<0.01),which was sig nificantly hig her than that of the control rice(63.1%)(P<0.05).Se-rich rice extract showed sig nif icant inhibition on EBV-EA in Raji cells.As the extract c oncentration was at 0.016μg /ml,0.078g /ml,and 0.388μg /ml,the inhibition rates of EA were2.85%,12.88%,and20.75%,respectively.CONCLUSION:The transformation of umbilical blo od B lymphocytes stimulated by EB virus a nd expression of EBV-EA in Raji cells may be sig nificantly inhibited by Se-rich rice extract,sug g esting that Se-rich rice can be used for preventing nasopharyng e al carcinoma.

关 键 词：富硒米 EB病毒 脐血 B淋巴细胞 细胞转化 肿瘤 EBV早期抗原 

分 类 号：R73-36[医药卫生—肿瘤]