胃良恶性病变中幽门螺杆菌感染与端粒酶的关系  

Relationship between H.pylori infection and telomerase activity in benign and malignant gastric lesions

作　　者：凌红[1] 陈惠新[1] 曾宏[1] 刘集鸿[1] 李韶光[1] 童志勇[1] 张育民[1] 

机构地区：[1]广东惠州市中心人民医院消化内科,516001

出　　处：《中国基层医药》2003年第1期21-23,共3页Chinese Journal of Primary Medicine and Pharmacy

基　　金：广东省医学科研课题资助(NO :1999591)

摘　　要：目的　探讨胃良恶性病变组织中端粒酶表达情况及其与幽门螺杆菌 (H .pylori)感染的关系。 方法　采用端粒酶TRAP ELISA方法检测 1 9例慢性浅表性胃炎 (CSG)、2 9例胃癌 (GC)、69例胃良性溃疡(GU)组织端粒酶活性 ,并分析三组H .pylori感染与端粒酶表达的关系。 结果　CSG、GU、GC三组端粒酶阳性表达率分别为 0 %、36 2 % (2 5/ 69)和 79 3 % (2 3/ 2 9)。其中GU组中无伴肠上皮化生及异型增生的 2 5例组织中端粒酶阳性率为 8% (2 / 2 5) ;GU伴肠上皮化生的为 40 % (6/ 1 5) ;GU伴异型增生的为 58 6 % (1 7/ 2 9)。CSG组与GC组及GU组比较、GU组与GC组比较 χ2 =2 4 6～ 9 6 ,P <0 0 1 ;GU无伴肠上皮化生及异型增生的与GU伴有肠上皮化生及异型增生的比较 χ2 =1 5 2 ,P <0 0 1 ;GU伴肠上皮化生的与GU伴异型增生的比较 χ2 =1 1 ,P >0 0 5。CSG、GU和GC组H .pylori感染率分别为 31 6 %、65 3 %和 44 8% ,CSG组与GC组比较、GU组与GC组比较差异无显著意义 (P >0 0 5) ;CSG组与GU组比较差异有显著意义 (χ2 =6 91 ,P<0 0 1 ) ,各组中H .pylori阳性组端粒酶阳性表达率与H .pylori阴性组端粒酶阳性表达率间比较差异均无显著意义 (P均 >0 0 5)。结论　端粒酶激活是胃癌发生的早期事件 ,但可能与H .pylori?Objective To investigate the relationship between telomerase activity and H.pylori infection in benign and malignant gastric lesions.Methods Twenty nine cases of gastric carcinoma(GC),69 benign gastric ulcers(GU),19 chronic superficial gastritis(CSG) were collected in the study.Their telomerase activity and H.pylori infection in the tissues were analyzed by TRAP ELISA and urease test,histological examination(Warthin Starry).Results The telomerase activities were 79 3%(23/29) in GC,36.2%(25/69) in GU and 0% in CSG,respectively.There were significant differences between CSG group and GC group and GU group,between GC group and GU group(χ 2=24.6～9.6,P<0.01).In GU group,the telomerase activities were 8%(2/25) in the GU without intestinal metaplasia(ICM) and dysplasia(Dys),40%(6/15) in the GU with ICM,58.6%(17/29) in the GU with Dys,respectively.There were significant differences between the GU without ICM and Dys and the GU with ICM and Dys(χ 2=15.2,P<0.01).There were no significant differences between the GU with ICM and the GU with Dys(χ 2=1.1,P>0.05)。H.pylori infection rates in GU,GC and CSG were 65.3%,44.8% and 31.6%,respectively,there were no significant differences between GU and GC groups(P>0.05),but it was higher in GU group than that in CSG group(χ 2=6.91,P<0.01).Among these groups,the telomerase activities were not significant differences between H.pylori positive patients and H.pylori negative patients(P>0.05).Conclusion Telomerase activity was highly expression in GC,but it may not be related to H.Pylori infection.

关 键 词：胃良性病变 胃癌 幽门螺杆菌 端粒酶 TRAP-ELISA 

分 类 号：R573.02[医药卫生—消化系统]