葡萄膜黑色素瘤多药耐药基因的表达与患者预后因素的分析  

Relationship of the gene of multidrug and drug resistance and the patients′ prognosis in uveal melanoma

作　　者：艾思明[1] 郭琳洁[2] 吴中耀[1] 张胜[1] 陈家祺[1] 郑湖玲[1] 

机构地区：[1]中山大学中山眼科中心,广州510060 [2]深圳市眼科医院

出　　处：《中华眼底病杂志》2003年第1期1-4,共4页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的　探讨葡萄膜黑色素瘤多药耐药基因的表达与患者预后的相关关系。　方法　选择石蜡包埋葡萄膜黑色素瘤组织 96例 ,通过脱色素免疫组织化学检测细胞周期素 D1(cyclin D1)、上皮生长因子受体(epithelial growth factor receptor,EGFR)、转移抑制基因 2 3(non- metastasis gene 2 3,nm 2 3)、P糖蛋白 (Pglucose protein,P- gp)、多药耐药相关蛋白 (multidrug resistance relation protein,MRP)、肺耐药蛋白 (lungresistance protein,L RP)基因表达。选择病例资料较完整者 ,进行随访 ,随访结果分类统计。　结果　 96例葡萄膜黑色素瘤中类上皮细胞型 2 1例、混合细胞型 5 6例和梭形细胞型 19例 ,其中眼内期 76例 ,眼外期 2 0例。随着转移抑制基因 nm 2 3表达的减低和 Cyclin D1、EGFR表达的增高 ,耐药相关基因表达有增高趋势 ,其中MRP和 L RP的表达与 nm 2 3的表达呈明显负相关关系 ,与 Cyclin D1、EGFR的表达呈明显正相关关系。随访结果显示 ,回访人数 5 8例 ,生存超过 5年者 2 6例 ,5年内死亡者 19例。结论　葡萄膜黑色素瘤多药耐药基因尤其 L RP的表达与患者预后具有明显的相关性。Objective To probe the relationship between the patients′ prognosis and the gene of multidrugs and drug resistance in uveal melanoma. Methods The gene expression of cyclin D1, epithelial growth factor receptor (EGFR), non metastasis gene 23 (nm 23), P glucose protein (P gp), multidrug resistance relation protein (MRP) and lung resistance protein (LRP) expression in 96 cases of uveal melanoma were detected by depigment immunohistochemistry. The patients with complete anamnesis data were observed continuously, and the follow up results were classified. Results Among the 96 cases of uveal melanoma, the epithelioid cell type was in 21, the mixed cell type in 56, and the spindle cell type in 19; including 76 at intraocular stage and 20 at extraocular stage. As the level of metastasis suppress gene nm 23 expression decreased and the level of cyclin D1 and EGFR expression increased, the expression level of drug resistance genes increased. The levels of LRP and MRP had negative correlation to the expressions of nm 23 and postive correlation to the expressions of nm 23, Cyclin D1 and EGFR. In 58 patients′ who were observed continuously, 19 died in 5 years and 26 survived over 5 years. Conclusion There are significant association between patients′ prognosis and multidrug and drug resistance gene in uveal melanoma.

关 键 词：葡萄膜黑色素瘤 多药耐药 葡萄膜肿瘤 药物疗法 药物耐受性 预后 基因表达 免疫组织化学 

分 类 号：R739.7[医药卫生—肿瘤]